食品科学 ›› 2009, Vol. 30 ›› Issue (21 ): 200-205.doi: 10.7506/spkx1002-6300-200921047

• 生物工程 • 上一篇    下一篇

不同碳源对黄伞菌丝生长的研究

张恒基1,黄清荣2 ,* ,常林瑞2,韩立亚2,欧秀元2   

  1. 1.鲁东大学绿化环卫科 2.鲁东大学生命科学学院
  • 收稿日期:2009-06-19 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 黄清荣 E-mail:ythqr801@163.com

Effect of Different Carbon Sources on Growth of Pholiota adipose

ZHANG Heng-ji1,HUANG Qing-rong2,*,CHANG Lin-rui2,HAN Li-ya2,OU Xiu-yuan2   

  1. 1. Department of Virescence and Environmental Sanitation, Ludong University, Yantai 264025, China;
    2. College of Life Science, Ludong University, Yantai 264025, China
  • Received:2009-06-19 Online:2009-11-01 Published:2010-12-29
  • Contact: HUANG Qing-rong E-mail:ythqr801@163.com

摘要:

采用平板培养和液体培养的方法研究不同碳源及其浓度对黄伞菌丝生长和胞外多糖产量的影响。结果表明:适宜黄伞菌平板培养菌丝的碳源及其浓度为:果糖 2.5%、麦芽糖3.0%、葡萄糖 3.0%、乳糖 3.0%、红糖2.5%、蔗糖2.5%;最适宜黄伞菌液体培养菌丝体的碳源为3.0% 的葡萄糖,最适宜黄伞菌产生胞外多糖的碳源为2.0% 的麦芽糖。

关键词: 黄伞, 碳源, 胞外多糖, 菌丝体得率

Abstract:

This study was designed to understand the effects of different carbon sources and its various concentrations on growth of Pholiota adiposa and exopolysaccharide production in plate culture and liquid culture. Results indicated that the optimal carbon sources and their concentrations for mycelia growth in plate culture were 3.0% fructose, 2.5% maltose, 3.0% glucose, 3.0% lactose, 2.5% brown sugar and 2.5% sucrose; glucose was determined to be the optimal carbon source for mycelia growth in liquid culture and its optimal concentration 3.0%; and the optimal carbon source for exopolysaccharide yield was maltose and its optimal concentration 2.0%.

Key words: Pholiota adiposa, carbon source, exopolysaccharide, mycelia yield

中图分类号: