食品科学 ›› 2009, Vol. 30 ›› Issue (21 ): 206-209.doi: 10.7506/spkx1002-6300-200921048

• 生物工程 • 上一篇    下一篇

小麦提取物对乳酸杆菌胆盐耐受力的影响

倪学勤1,2,曾 东1,2,*,张 洪1,田贵平1,但 言1   

  1. 1.四川农业大学动物医学院动物微生态研究中心 2.动物疫病与人类健康四川省重点实验室
  • 收稿日期:2009-07-15 出版日期:2009-11-01 发布日期:2010-12-29
  • 通讯作者: 曾 东 E-mail:zend@sicau.edu.cn
  • 基金资助:

    教育部“长江学者和创新团队发展计划”项目(IRT0848;IRT0555);四川省教育厅应用基础项目(07ZA065)

Effect of Wheat Extracts on Bile Tolerance of Selected Strains of Lactobacilli

NI Xue-qin1,2,ZENG Dong1,2,*,ZHANG Hong1,TIAN Gui-ping1,DAN Yan1   

  1. 1. Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China;
    2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya'an 625014, China
  • Received:2009-07-15 Online:2009-11-01 Published:2010-12-29
  • Contact: ZENG Dong E-mail:zend@sicau.edu.cn

摘要:

模拟人十二指肠高胆盐环境,研究小麦提取物对乳酸杆菌胆盐耐受力的影响,探讨作为生产益生菌食品的小麦基质对乳酸杆菌的保护作用。将约氏乳酸杆菌JJB3、SNZ10,罗伊氏乳酸杆菌MNZ8、KNZ4 株菌,在含有小麦提取物的10g/L 胆盐溶液,pH7.0 的磷酸盐缓冲液中作用4h,检测其活菌数。未添加小麦提取物的对照4 株乳酸杆菌活菌数显著下降(P < 0.05),其中MNZ8 对胆盐最为敏感,活菌数下降1.74 lg(CFU/ml),KNZ4 的耐受力最强,活菌数下降1.16 lg(CFU/ml);添加小麦提取物后,4 株乳酸杆菌的胆盐耐受力均显著提高(P < 0.05),但对不同菌株提高程度有差异,其中,JJB3 胆盐耐受力提高最明显,其活菌仅减少了0.11 lg(CFU/ml)。葡萄糖和游离氨基氮(FAN)的浓度与乳酸杆菌耐胆盐能力的提高呈正相关关系,但葡萄糖的保护作用显著高于游离氨基氮。小麦提取物对4 株乳酸杆菌耐受模拟十二指胆盐环境具有较好的保护作用,对约氏乳酸杆菌JJB3 保护作用最好。

关键词: 小麦, 提取物, 乳酸杆菌, 胆盐耐受力

Abstract:

In order to explore the protection effect of wheat providing the matrix for production of probiotic foods on Lactobacilli, the effect of wheat extracts on viability of selected stains of Lactobacilli, including Lactobacillus johnsonii JJB3, SNZ10, and Lactobacillus reuteri MNZ8, KNZ4 was investigated under the circumstance of simulated bile juice. The viability of these strains was detected during exposure to 10 g/L bile for 4 h at pH 7.0. In additions, the effects of glucose and free amino nitrogen (FAN) on their bile tolerance with increasing concentration were also examined. In the absence of wheat extracts KNZ4 showed the greatest resistance to bile with a reduction of viable count of only 1.16 lg (CFU/ml), whilst MNZ8 displayed the greatest sensitivity with a reduction of viable count of up to 1.74 lg (CFU/ml). Addition of wheat extracts significantly improved the tolerance of all four strains (p<0.05), and JJB3 showed the highest bile tolerance and the viable population was decreased by 0.11 lg(CFU/ml). The extent of improvement was positively correlated with the concentration of soluble sugars and FAN present in the extracts. Glucose revealed a better effect than FAN. The results presented in this study indicate that wheat extracts exhibit a significant protective effect on the viability of four Lactobacilli strains under simulated bile conditions, especially for L. johnsonii JJB3.

Key words: wheat, extracts, Lactobacilli, bile tolerance

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