食品科学 ›› 2009, Vol. 30 ›› Issue (12): 40-42.doi: 10.7506/spkx1002-6630-200912003

• 工艺技术 • 上一篇    下一篇

水解猪肉蛋白制备食用香精的工艺研究

赵妍嫣,姜绍通,陈 雄   

  1. 合肥工业大学生物与食品工程学院
  • 收稿日期:2008-09-01 修回日期:2008-12-02 出版日期:2009-06-15 发布日期:2010-12-29
  • 通讯作者: 赵妍嫣 E-mail:zhaoyy33@163.com
  • 基金资助:

    安徽省科技攻关计划项目(08010302209)

Use of Maillard Reaction for the Preparation of Food Flavor from Hydrolyzed Pork Protein

ZHAO Yan-yan,JIANG Shao-tong,CHEN Xiong   

  1. (College of Biology and Food Engineering, Hefei University of Technology, Hefei 230069, China)
  • Received:2008-09-01 Revised:2008-12-02 Online:2009-06-15 Published:2010-12-29
  • Contact: ZHAO Yan-yan E-mail:zhaoyy33@163.com

摘要:

以猪肉为原料,通过Maillard 反应制备肉味香精。研究了初始pH 值、温度、蛋白酶的添加量及水解时间对制备动物水解蛋白(HAP)的影响。获得的最佳水解工艺条件为:pH7.5,温度60℃,加酶0.9%,水解3h,在此条件下,蛋白水解度达到11.56%。以此水解液作为Maillard 反应的底物,应用感官分析法研究了pH 值、反应时间、温度以及HAP 添加量对Maillard 反应制备食用香精的影响,获得的最优工艺条件为:pH8.5,HAP 添加量60%,温度120℃,反应40min。在此条件下,应液产生了香气较强而且协调的肉香味。

关键词: 动物蛋白, 水解, Maillard反应, 香精

Abstract:

Pork protein was hydrolyzed by animal proteolysis enzyme, and then the hydrolysate was used to prepare food flavor by means of Maillard reaction. The hydrolysis and Maillard reaction conditions were optimized by orthogonal test with degree of hydrolysis and sensory evaluation score as the indicators, respectively. The optimum initial pH value, temperature, enzyme dose, and duration for hydrolysis were 7.5, 60 ℃, 0.9%, and 3 h, and under these conditions, the degree of hydrolysis was 11.56%. Under optimized conditions for Maillard reaction (pH 8.5, pork protein hydrolysate amount 60%, 120 ℃, and reaction 40 min), the product obtained had the highest sensory evaluation score, and it was of full-bodied and harmonious meat flavor.

Key words: animal protein, hydrolyzing, Maillard reaction, flavor

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