食品科学 ›› 2009, Vol. 30 ›› Issue (14 ): 309-313.doi: 10.7506/spkx1002-6630-200914069

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采后番茄果实抗氧化酶活与抗冷性的关系

赵丹莹,申 琳,于萌萌,郑 杨,生吉萍*   

  1. 中国农业大学食品科学与营养工程学院
  • 收稿日期:2009-03-30 出版日期:2009-07-15 发布日期:2010-12-29
  • 通讯作者: 生吉萍* E-mail:pingshen@cau.edu.cn
  • 基金资助:

    国家自然科学基金项目(30571296;30671471)

Relationship between Activities of Antioxidant Enzymes and Clod Tolerance of Postharvest Tomato Fruits

ZHAO Dan-ying,SHEN Lin,YU Meng-meng,ZHENG Yang,SHENG Ji-ping*   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2009-03-30 Online:2009-07-15 Published:2010-12-29
  • Contact: SHENG Ji-ping* E-mail:pingshen@cau.edu.cn

摘要:

为了探讨具有不同冷敏感性的番茄采后果实中抗氧化酶活与抗冷性的关系,寻找果实抗冷性评价指标,本研究对具有不同冷敏性的两个番茄品种,Santiam(Solanum lycopersicum L. cv. Santiam)和丽春(Solanum lycopersicum L.cv. Lichun)采后果实进行短时(24h)及长期(20d)低温胁迫处理。检测期间与冷害程度密切相关的四种抗氧化酶,过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性水平及变化趋势,发现四种酶的活性反映了品种抗冷性,二者存在正相关性。且通过对比证明,4~24h 的短时低温胁迫下,品种间CAT、POD 和SOD 的活性高低便可显著反映抗冷性的差异(p<0.05)。说明利用抗氧酶活性评价采后果实抗冷性的方法具有一定的灵敏性及可行性。

关键词: 抗氧化酶, 冷害, 抗冷性, 氧化胁迫, 番茄果实

Abstract:

In order to find the relationship between chilling sensitivity and activities of antioxidant enzymes of postharvest tomato fruits, two cultivars of postharvest tomato fruits, Santiam (Solanum lycopersicum L. cv. Santiam) and Lichun (Solanum lycopersicum L. cv. Lichun), were treated with short-time (24 h) or long-time (20 d) chilling stress, and the changes in activities of antioxidant enzymes such as catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and superoxide dismutase (SOD) during chilling stress were measured. It was found that chilling tolerance of tomato cultivars could be indicated obviously by activities of the four enzymes, and a positive relationship was observed between them. Activities of CAT, POD and SOD could distinctively reflect chilling tolerance diversity (p < 0.05) between the two cultivars within 4-24 h under chilling stress. Conclusively, cold tolerance of postharvest tomato fruits can be fast and effectively judged from activities of the antioxidant enzymes.

Key words: antioxidant enzyme, chilling injury, cold tolerance, oxidative stress, tomato fruit

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