转基因库拉索芦荟多糖的分离纯化与结构表征

doi:10.7506/spkx1002-6630-200915002

食品科学 ›› 2009, Vol. 30 ›› Issue (15): 17-20.doi: 10.7506/spkx1002-6630-200915002

转基因库拉索芦荟多糖的分离纯化与结构表征

赵华¹,赵进²,何聪芬²,董银卯²,钟秦¹

1. 南京理工大学化工学院 2. 北京工商大学化学与环境工程学院

收稿日期:2009-01-08 修回日期:2009-05-22 出版日期:2009-08-01 发布日期:2010-12-29
通讯作者: 董银卯 E-mail:dongym@th.btbu.edu.cn
基金资助:
国家“863”计划项目(2007AA100404)；北京市教委、北京市基金委科技重点项目(Kz200410011006)

Extraction, Purification and Structural Characterization of Polysaccharide from Transgenic Aloe vera L.

ZHAO Hua1,2，ZHAO Jin2，HE Cong-fen2，DONG Yin-mao2,*，ZHONG Qin1

1. School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China；
2. College of Chemistry and Environment Engineering, Beijing Technology and Business University, Beijing 100048, China

Received:2009-01-08 Revised:2009-05-22 Online:2009-08-01 Published:2010-12-29
Contact: DONG Yin-mao2,*， E-mail:dongym@th.btbu.edu.cn

摘要/Abstract

摘要：

以3 年生转基因的库拉索芦荟凝胶为材料，冷处理法醇沉提取粗多糖AP-1，苯酚- 硫酸法检测糖含量为57.3%，考马斯亮蓝染色法检测蛋白质含量为2.892%。经IR 鉴定，AP-1 为含有乙酰化β-D- 吡喃甘露聚糖的混合物；质谱测定表明，分子量测定范围在12900D 和13100D。纯化后所得多糖半精品AP-2 为白色粉末，糖含量比AP-1 提高9.6%。用DEAE Sephadex A-25 分级分离AP-2，得到中性糖(AP-3)和酸性糖(AP-4)。用Sepharose CL-6B凝胶分离AP-3，得到AP-5 和AP-6。用IR、MS、GC 对AP-1、AP-2、AP-3、AP-4、AP-5、AP-6 进行鉴定和表征分析，结果表明，中性糖AP-3 为多甘露糖型的多糖，并推测AP-5 生物活性较AP-6 高。

关键词: 转基因库拉索芦荟, 芦荟多糖, 分离纯化, 结构表征

Abstract:

Aloe has been used in Chinese traditional medicine for over two thousand of years. Polysaccharides as main active components in Aloe vera L. have aroused much interest. Aloe polysaccharide (AP-1) was extracted from fresh leaves of threeyear- old transgenic Aloe vera L. by cold ethanol precipitation. The amount of total carbohydrates in AP-1 was measured by phenolsulfuric acid method to be 57.3%. AP-1 was proved to be a mixture of acetylated β-D-pyranmannans and the molecular weight was within the range of 12900－13100 dalton detected by IR spectrometry analysis. The amount of total carbohydrates in AP- 2 was increased by 9.6% when compared with that in AP-1 after purification by Sevag method. Neutral polysaccharide (AP-3) and acidic polysaccharide (AP-4) were obtained from AP-2 by DEAE Sephadex A-25 and AP-5 and AP-6 were obtained from AP-3 by Sepharose CL-6B. The monosaccharide composition analysis indicated that AP-3 was structurally identified as a polymannan type polysaccharide and the bioactivity of AP-5 was deduced to be higher than that of AP-6.

Key words: transgenic Aloe vera L., aloe polysaccharide, extraction and purification, structure characterization

中图分类号:

Q539

赵华1,2，赵进2，何聪芬2，董银卯2,*，钟秦1. 转基因库拉索芦荟多糖的分离纯化与结构表征[J]. 食品科学, 2009, 30(15): 17-20.

ZHAO Hua1,2，ZHAO Jin2，HE Cong-fen2，DONG Yin-mao2,*，ZHONG Qin1. Extraction, Purification and Structural Characterization of Polysaccharide from Transgenic Aloe vera L.[J]. FOOD SCIENCE, 2009, 30(15): 17-20.

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转基因库拉索芦荟多糖的分离纯化与结构表征

Extraction, Purification and Structural Characterization of Polysaccharide from Transgenic Aloe vera L.

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