食品科学 ›› 2009, Vol. 30 ›› Issue (24 ): 38-41.doi: 10.7506/spkx1002-6630-200924005

• 工艺技术 • 上一篇    下一篇

还原剂- 碱法复合提取大米蛋白的工艺研究

刘纯友,刘永乐* ,俞 健,易翠平,李向红   

  1. 长沙理工大学化学与生物工程学院
  • 收稿日期:2009-07-01 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 刘永乐* E-mail:lyle19@163.com
  • 基金资助:

    国家高技术研究发展计划项目(2008AA100801);湖南省科技重大专项(2008FJ1004)

Protein Extraction from Rice Endosperm Using Reductant-alkali Method

LIU Chun-you,LIU Yong-le*,YU Jian,YI Cui-ping,LI Xiang-hong   

  1. College of Chemistry and Biology Engineering, Changsha University of Science and Technology,
    Changsha 410004, China
  • Received:2009-07-01 Online:2009-12-15 Published:2010-12-29
  • Contact: LIU Yong-le*, E-mail:lyle19@163.com

摘要:

以早籼米为原料,用还原剂配合碱溶酸沉的方法提取大米蛋白。通过单因素试验与正交试验得出优化的提取条件为碱液浓度0.05mol/L、Na2SO3 用量为米粉的0.5%、DTT 用量为米粉的2.5%、固液比1:12(g/ml)、室温提取4h,在此条件下,大米蛋白提取率可达95.26%。

关键词: 大米蛋白, 还原剂, 碱溶液, 提取

Abstract:

In this study, a combinatorial method of reductant-alkali solution and acid precipitation was used to extract protein from rice endosperm. The optimal condition for rice protein extraction was determined through single factor and orthogonal experiments to be 0.05 mol/L sodium hydroxide, 0.50% sodium sulfite, 2.50% dithiothreitol, 1:12 (g/ml) of solid-liquid ratio, and 4 h of extraction time. Extraction rate of rice protein was 95.26% under this optimal condition.

Key words: rice protein, reductant, alkali solution, extraction

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