食品科学 ›› 2010, Vol. 31 ›› Issue (12): 67-70.doi: 10.7506/spkx1002-6630-201012015

• 工艺技术 • 上一篇    下一篇

纤维素酶提取湘西椪柑皮中总黄酮的工艺优化研究

欧阳辉,余 佶,张永康   

  1. 吉首大学 植物资源保护与利用湖南省高校重点实验室
  • 收稿日期:2009-11-05 修回日期:2010-02-01 出版日期:2010-06-15 发布日期:2010-12-29
  • 通讯作者: 欧阳辉 E-mail:oyhmail@163.com
  • 基金资助:

    湖南省教育厅科研基金项目(09C786);湘西州科技计划项目(州科字[2009]43 号)

Extraction of Total Flavonoids from the Peel of Ponkan Mandarin (Citrus reticulata Blanco) by Optimized Cellulase Hydrolysis

OUYANG Hui,YU Ji,ZHANG Yong-kang   

  1. Hunan Provincial University Key Laboratory of Plant Resources Conservation and Utilization, Jishou University,
    Jishou 416000, China
  • Received:2009-11-05 Revised:2010-02-01 Online:2010-06-15 Published:2010-12-29
  • Contact: OUYANG Hui, E-mail:oyhmail@163.com

摘要:

采用响应面法对纤维素酶提取椪柑皮中总黄酮的工艺进行优化。结果表明,酶法提取椪柑皮总黄酮的最佳工艺条件为酶用量120U/mL、酶解温度52℃、酶解时间140min、酶解pH4.35,总黄酮得率可达1.85%。

关键词: 椪柑皮, 总黄酮, 纤维素酶, 响应面法, 提取

Abstract:

The purpose of the present study was to investigate the use of response surface methodology based on Box-Benhnken experimental design to optimize the cellulase hydrolysis of Ponkan mandarin peel for the extraction of total flavonoids. The optimum hydrolysis conditions for the extraction of total flavonoids were determined to be: enzyme concentration 120 U/mL, hydrolysis temperature 52 ℃, length of hydrolysis time 140 min and pH 4.35. Under such conditions, the extraction yield of total flavonoids reached up to 1.85%.

Key words: Ponkan mandarin peel, total flavonoids, cellulase, response surface methodology (RSM), extraction

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