食品科学 ›› 2010, Vol. 31 ›› Issue (18): 278-282.doi: 10.7506/spkx1002-6630-201018065

• 分析检测 • 上一篇    下一篇

牦牛乳软质干酪成熟期挥发性风味成分分析

牛 婕1,甘伯中1,*,乔海军2,刘兴龙3   

  1. 1.甘肃农业大学食品科学与工程学院 2.甘肃农业大学理学院 3.甘肃华羚生物科技有限公司
  • 收稿日期:2009-12-22 修回日期:2010-06-11 出版日期:2010-09-25 发布日期:2010-12-29
  • 通讯作者: 牛婕 E-mail:niujie-nj@163.com
  • 基金资助:

    教育部科学技术研究重点项目(210225);甘肃省高等学校研究生导师科研项目(1002-12)

Analysis of Volatile Compounds in Yak s Milk Soft Cheese during Ripening

NIU Jie1,GAN Bo-zhong1,*,QIAO Hai-jun2,LIU Xing-long3   

  1. 1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China;2. College of Science, Gansu
    Agricultural University, Lanzhou 730070, China ;3. Gansu Hualing Biotechnology Co. Ltd., Lanzhou 730000, China
  • Received:2009-12-22 Revised:2010-06-11 Online:2010-09-25 Published:2010-12-29
  • Contact: NIU Jie1 E-mail:niujie-nj@163.com

摘要:

采用固相微萃取- 气相色谱- 质谱联用(SPME-GC-MS)对不同成熟时期(30、60、90d)牦牛乳软质干酪挥发性风味物质进行分离鉴定,共检测出45 种化合物,并用峰面积归一化法确定各种化合物的相对百分含量。通过对固相微萃取纤维头和萃取温度的筛选优化实验条件,采用75μm CAR/PDMS(碳分子筛- 聚二甲基硅氧烷)萃取纤维头,50℃条件下吸附不同成熟时期(30、60、90d)干酪的挥发性风味物质效果较好。检测出的挥发性风味物质主要是酸类、醇类、酮类,其次为酯类和醛类化合物,酸类物质构成了牦牛乳软质干酪的主体特征风味。乙酸、丁酸、己酸成为干酪中的优势风味物质。

关键词: 牦牛乳软质干酪, 风味物质, 固相微萃取(SPME), 气相色谱-质谱法(GC-MS)

Abstract:

The flavor components in different ripening periods (30, 60 d and 90 d) in yak s milk soft cheese were extracted by solid phase micro-extraction (SPME) and identified by gas chromatography-mass spectrometry (GC-MS) analysis. A total of 45 compounds were detected and relative contents of these compounds were determined by peak area normalization method. The conditions of SPME were optimized to be 75μm CAR/PDMS and temperature of 50 ℃. The major volatile flavor compounds in yak s milk soft cheese were acids, alcohols and ketones. Esters and aldehydes were also detected as the volatile flavor components. Acidic compounds were the major flavor components of yak s milk soft cheese, among which, acetic acid, butyric acid, caproic acid and 2-heptanone were the most predominant compounds.

Key words: yak s milk soft cheese, flavor component, solid phase micro-extraction (SPME), gas chromatography-mass spectrometry (GC-MS)

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