食品科学 ›› 2011, Vol. 32 ›› Issue (15): 70-73.doi: 10.7506/spkx1002-6630-201115017

• 基础研究 • 上一篇    下一篇

花椒黄酮抗氧化作用研究

张宇思,龚祝南,石雪萍   

  1. 1.南京师范大学生命科学学院 2.南京野生植物综合利用研究院
  • 出版日期:2011-08-15 发布日期:2011-07-26
  • 基金资助:
    “十一五”国家科技支撑计划项目(2006BAD06B02)

Antioxidant Effect of Total Flavonoids from Zanthoxylum bungeanum Seeds

ZHANG Yu-si1,GONG Zhu-nan1,SHI Xue-ping2,*   

  1. (1. College of Life Science, Nanjing Normal University, Nanjing 210097, China; 2. Nanjing Institute for Comprehensive Utilization of Wild Plant, Nanjing 210042, China)
  • Online:2011-08-15 Published:2011-07-26

摘要: 采用60%乙醇提取花椒总黄酮并用AB-8型大孔吸附树脂纯化。通过DPPH自由基实验、总还原力实验、总抗氧化能力实验和脂质抗氧化实验研究花椒总黄酮的体外抗氧化作用。结果表明:的花椒总黄酮纯化样对DPPH自由基清除效果强于同质量浓度的BHT,略低于VC;花椒总黄酮初提物和纯化样的还原能力都随其质量浓度的增加而明显增大;初提物和纯化样的总抗氧化能力与其质量浓度都呈正相关性,随着质量浓度增大,总抗氧化能力也增加;花椒总黄酮初提物和纯化样对Fe2+引发的卵磷脂脂质体过氧化有抑制作用,抑制率随着质量浓度的增加而缓慢增大;另外,纯化后的样品要比初提物的样品抗氧化作用更强。

关键词: 花椒, 黄酮, 抗氧化作用

Abstract: The antioxidant effect of crude total flavonoid extract from Zanthoxylum bungeanum seeds obtained by extraction with 60% ethanol aqueous solution and its purified counterpart obtained by adsorption on type AB-8 macroporous resin was evaluated by DPPH radical scavenging, total reducing power, total antioxidant capacity and anti-lipoperoxidation assays. The results showed that purified total flavonoids from Zanthoxylum bungeanum seeds had stronger antioxidant effect than BHT at the same concentrations. Crude and purified total flavonoids from Zanthoxylum bungeanum seeds indicated a noticeable increase with increasing concentration and a positive correlation between their total antioxidant activity and concentration. Crude and purified total flavonoids from Zanthoxylum bungeanum seeds could inhibit lecithin phospholipid liposome peroxidation induced by Fe2+ in a concentration-dependent fashion. In addition, crude total flavonoid extract from Zanthoxylum bungeanum seeds showed higher antioxidant effect than its purified counterpart.

Key words: Zanthoxylum bungeanum, flavonoids, antioxidant activity

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