北虫草体外抗病毒、抗肿瘤活性部位的筛选

doi:10.7506/spkx1002-6630-201117065

食品科学 ›› 2011, Vol. 32 ›› Issue (17): 313-316.doi: 10.7506/spkx1002-6630-201117065

北虫草体外抗病毒、抗肿瘤活性部位的筛选

杜秀菊1,2，张劲松2,*，蔡连捷3，刘艳芳2，贾薇2，唐庆久2，杨焱2，周帅2

1. 聊城大学生命科学学院
2.上海市农业科学院天然药用资源研究开发中心，国家食用菌加工技术分中心，上海市农业科学院食用菌研究所 3.聊城大学资产处

发布日期:2011-08-30
基金资助:
“十一五”国家科技支撑计划项目(2006BAD06B08)

Antitumor and Antiviral Activities of Extracts from Fruiting Bodies of Codyceps militaris

DU Xiu-ju1,2， ZHANG Jin-song2,*，CAI Lian-jie3， LIU Yan-fang2， JIA Wei2， TANG Qing-jiu2，YANG Yan2， ZHOU Shuai2

(1. College of Life Science, Liaocheng University, Liaocheng 252059, China；2.Research Center of Natural Medicinal Resource of Shanghai Academy of Agricultural Sciences, National R&D Center for Edible Fungi Processing, Edible Fungi Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China； 3.Asset Office, Liaocheng University, Liaocheng 252059, China)

Published:2011-08-30

摘要/Abstract

摘要： 目的：采用系统溶剂法对北虫草子实体提取分离，并对各提取部位进行抗病毒和抗肿瘤活性研究。方法：采用CPE法进行体外抗病毒(抗流感甲型病毒和抗疱疹病毒)实验，按Reed-Muench法计算各提取部位的药物半数有毒质量浓度(TC50)、半数抑制浓度(IC50)和选择指数(SI)，观察各提取部位的抗病毒活性；用Alamar Blue法，采用体外肿瘤细胞株K562和MCF-7的增殖抑制实验模型，研究北虫草子实体的抗肿瘤活性。结果：抗病毒实验结果表明，氯仿相(CMCl)具有抗疱疹病毒HSV-Ⅰ和 HSV-Ⅱ的活性，CMCl对HSV-Ⅰ和HSV-Ⅱ病毒的半数抑制质量浓度(IC50)分别为194.01μg/mL和173.34μg/mL，选择指数(SI)分别为：＞1.29和＞1.44；3个部位均没有抗流感甲型病毒活性。抗肿瘤实验结果表明，北虫草3个部位提取物，在中、高质量浓度(50、200μg/mL)时，对抑制K562的增殖都有一定的活性，活性高低为：乙醇相(CMEtOH)＞氯仿相(CMCl)＞乙酸乙酯相(CMEtOAc)；3个部位相比较，仅CMCl对人乳腺癌细胞株MCF-7具有抑制活性，在10、50、200μg/mL的抑制率分别为(9.6±3.0)%、(46.1±8.9)%和(68.5±9.2)%，呈现明显的质量浓度依赖性。结论：在北虫草3个部位中，CMCl是抗肿瘤和抗病毒活性较高的活性部位，值得进一步开发利用。

关键词: 北虫草, 抗肿瘤, 抗病毒, 系统溶剂法

Abstract: Objective: To extract and isolate bioactive components from the fruiting bodies of Codyceps militaris by systematic solvent method and determine the antitumor and antiviral activities of chloroform extract from the fruiting bodies of Codyceps militaris and its fractions soluble in ethyl acetate and ethanol. Methods: The antiviral activities of extracts on FLU-A, HSV-I and HSV-II viruses were assessed by cytopathic effect (CPE) method. The 50% toxic concentration (TC50), 50% inhibitory concentration (IC50) and selective index (SI) of the chloroform extract and its two fractions were determined by Reed-Muench method. Their antitumor activities were evaluated based on their inhibitory rates on tumor cell proliferation such as K562 and MCF-7 cells, determined by Alamar Blue method. Results: The antiviral activity assay showed that the chloroform extract had the best antiviral activity on HSV-I and HSV-II viruses with an IC50 of 194.01μg/mL and 173.34μg/mL, and an SI of higher than 1.29 and 1.44, respectively. The chloroform extract and its two fractions had no inhibitory effect on FLU-A virus but had obvious antitumor activity on K562 cells at the concentrations of 50 μg/mL and 200 μg/mL in and the ethanol-soluble fraction ranked first in decreasing order of anti-K562 cell activity, followed by the chloroform extract and its ethyl acetate-soluble fraction. Only the chloroform extract revealed an inhibitory effect on MCF-7 cells with inhibitory rates of (9.6 ± 3.0)%, (46.1 ± 8.9)%, and (68.5 ± 9.2)% at the concentrations of 10, 50μg/mL and 200 μg/mL, respectively, in a dose-dependent manner. Conclusion: The chloroform extract has higher antitumor and antiviral activities than its two fractions so that it will provide a potential resource for natural antitumor and antiviral agents.

Key words: Codyceps militaris, antitumor activity, antiviral activity, systematic solvent extraction

中图分类号:

R932
R936

杜秀菊，张劲松，蔡连捷，刘艳芳，贾薇，唐庆久，杨焱，周帅. 北虫草体外抗病毒、抗肿瘤活性部位的筛选[J]. 食品科学, 2011, 32(17): 313-316.

DU Xiu-ju， ZHANG Jin-song，CAI Lian-jie， LIU Yan-fang， JIA Wei，TANG Qing-jiu，YANG Yan， ZHOU Shuai. Antitumor and Antiviral Activities of Extracts from Fruiting Bodies of Codyceps militaris[J]. FOOD SCIENCE, 2011, 32(17): 313-316.

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北虫草体外抗病毒、抗肿瘤活性部位的筛选

Antitumor and Antiviral Activities of Extracts from Fruiting Bodies of Codyceps militaris

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