食品科学 ›› 2011, Vol. 32 ›› Issue (22): 16-19.doi: 10.7506/spkx1002-6630-201122004

• 工艺技术 • 上一篇    下一篇

L-半胱氨酸处理对玉米醇溶蛋白提取的影响

徐艳艳1,李梦琴1,*,任红涛1,蔡花真2   

  1. 1.河南农业大学食品科学技术学院 2.河南质量工程职业学院
  • 出版日期:2011-11-25 发布日期:2011-11-11
  • 基金资助:
    河南省教育厅自然科学研究计划项目(2010A550008);河南农业大学重点学科资助项目

Effect of L-cysteine Treatment on Zein Extraction

XU Yan-yan1,LI Meng-qin1,*,REN Hong-tao1,CAI Hua-zhen2   

  1. (1. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China; 2. Henan Quality Engineering Vocational College, Pingdingshan 467000, China)
  • Online:2011-11-25 Published:2011-11-11

摘要: 在单因素试验的基础上,通过正交试验优化玉米醇溶蛋白(zein)的提取工艺,研究L-半胱氨酸用量、液料比、超声波时间、超声波温度、pH值因素对玉米醇溶蛋白提取率的影响,并确定最佳工艺条件为L-半胱氨酸用量0.65%、液料比7:1、超声波时间1.5h、超声波温度40℃、pH5.0,在此条件下得到最终玉米醇溶蛋白提取率为89.06%,纯度为92.51%。

关键词: L-半胱氨酸, 玉米醇溶蛋白, 提取

Abstract: Based on one-factor-at-a-time experiments, process optimization for zein extraction using L-cysteine treatment followed by ultrasonic-assisted extraction was performed using an orthogonal array design. Zein extraction yield was investigated with respect to L-cysteine amount, ratio of liquid to material, ultrasonic treatment time, temperature and pH. The optimal process conditions for zein extraction were achieved as follows: L-cysteine amount 0.65%, ratio of liquid to material 7:1, ultrasonic treatment time 1.5 h, temperature 40 ℃, and pH 5.0. Under these conditions, the yield and purity of zein were 89.06% and 92.51%, respectively.

Key words: L-cysteine, zein, extraction

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