薏米中酚类提取物测定及抗氧化能力指数分析

doi:10.7506/spkx1002-6630-201201014

食品科学 ›› 2012, Vol. 33 ›› Issue (1): 72-76.doi: 10.7506/spkx1002-6630-201201014

薏米中酚类提取物测定及抗氧化能力指数分析

王立峰1,2，何荣1，袁建2，鞠兴荣2

1.江南大学食品学院，江苏无锡 214122；2.南京财经大学食品科学与工程学院，江苏南京 210046

收稿日期:2011-11-25 修回日期:2012-01-09 出版日期:2012-01-15 发布日期:2012-01-12
通讯作者: 王立峰 E-mail:wanglifeng_8@163.com
基金资助:
江苏省“青蓝工程”青年骨干教师项目；江苏省政府留学奖学金项目

Analysis of Phenols, Flavonoids and Oxygen Radical Absorbance Capacity (ORAC) of Three Varieties of Adlay

WANG Li-feng，HE Rong，YUAN Jian，JU Xing-rong

1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China；
2. School of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, China

Received:2011-11-25 Revised:2012-01-09 Online:2012-01-15 Published:2012-01-12
Contact: Li-Feng WANG E-mail:wanglifeng_8@163.com

摘要/Abstract

摘要：

选取3种薏米样品，经提取获得游离型和结合型多酚类化合物，采用改进的Folin-Ciocalteu比色法及硼氢化钠/氯醌(SBC)比色法，用酶标仪测定其多酚和黄酮含量；采用抗氧化能力指数(ORAC)方法，于荧光酶标仪中测定提取物中的总抗氧化活性。研究表明，游离型多酚含量中龙薏1号薏米显著性高于贵州黑谷薏米和辽宁5号薏米，结合型多酚含量中龙薏1号薏米显著性高于贵州黑米薏米和辽宁5号薏米，所有样品中其平均结合型多酚含量占总多酚含量的45.28%；游离型黄酮含量中辽宁5号薏米显著高于贵州黑谷薏米和辽宁5号薏米，结合型黄酮含量中龙薏1号薏米高于辽宁5号薏米和贵州黑谷薏米，总黄酮含量最高的是辽宁5号薏米。总抗氧化能力指数(ORAC)最高的是龙薏1号薏米，其值是(668.0 ± 32.7)mg Trolox /100g样品(以干质量计)，其次是贵州黑谷薏米和辽宁5号薏米，所有样品中平均结合型ORAC值占总ORAC值的48.08%；3个品种的薏米其总多酚含量与总抗氧化能力指数之间相关系数为0.933，有显著相关性(P＜0.05)。

关键词: 薏米, 多酚, 黄酮, 抗氧化能力指数

Abstract:

In order to explore the phytochemical profile of three adlay varieties, the levels of total, free and bound phenols and flavonoids were determined using a microplate reader. Meanwhile, the oxygen radical absorbance capacity (ORAC) was measured as an indicator for total antioxidant capacity using a multifunctional fluorescence microplate reader. The contents of free ((45.19 ± 0.91) mg of gallic acid equivalent/100 g of dried samples) and bound ((30.86 ± 1.18) mg of gallic acid equivalent/100 g samples) phenols in Longyi I were significantly higher than those in Liaoning V and Guizhou Heigu (P ＜ 0.05). For all these adlay varieties, bound phenols averagely accounted for 45.28% of total phenols. The content of free flavonoids in Liaoning V showed a significant increase as compared with Longyi I and Guizhou Heigu, and the content of bound flavonoids in Longyi I was higher than that of Liaoning V and Guizhou Heigu. The highest content of total flavonoids was found in Liaoning V. The adlay varieties were ranked in decreasing order of ORAC as follows: Longyi I ((668.0 ± 32.7) mg of Trolox equivalent/100 g of dried samples), Liaoning V and Guizhou Heigu. The average percentage of bound ORAC in total ORAC was 48.08%. A correlation coefficient (r2) of 0.933 was observed for the significant correlation (P ＜ 0.05) between the total phenol content and total antioxidant capacity of each adlay variety.

Key words: adlay, phenols, flavonoids, oxygen radical absorbance capacity(ORAC)

中图分类号:

TS201.1

王立峰，何荣，袁建，鞠兴荣. 薏米中酚类提取物测定及抗氧化能力指数分析[J]. 食品科学, 2012, 33(1): 72-76.

WANG Li-feng，HE Rong，YUAN Jian，JU Xing-rong. Analysis of Phenols, Flavonoids and Oxygen Radical Absorbance Capacity (ORAC) of Three Varieties of Adlay[J]. FOOD SCIENCE, 2012, 33(1): 72-76.

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薏米中酚类提取物测定及抗氧化能力指数分析

Analysis of Phenols, Flavonoids and Oxygen Radical Absorbance Capacity (ORAC) of Three Varieties of Adlay

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