食品科学 ›› 2012, Vol. 33 ›› Issue (13): 66-69.doi: 10.7506/spkx1002-6630-201213012

• 基础研究 • 上一篇    下一篇

不同超声频率对温州蜜柑皮总酚和抗氧化能力的影响

马亚琴,吴厚玖,周志钦,王华,孙志高   

  1. 1.西南大学温州蜜柑研究所,国家温州蜜柑工程技术研究中心 2.西南大学园艺园林学院
  • 出版日期:2012-07-15 发布日期:2012-07-27
  • 基金资助:
    重庆市自然科学基金计划项目(2011jjA80030);中央高校基本科研业务费专项(100030-2120130719)

Effect of Different Ultrasonic Frequencies on Total Phenolic Content and Antioxidant Capacity of Methanol Extracts from Citrus unshiu Peels

MAYa-qin,WUHou-jiu,ZHOUZhi-qin,WANGHua,SUNZhi-gao   

  1. (1. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing 400712, China; 2. College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400712, China)
  • Online:2012-07-15 Published:2012-07-27

摘要: 对比分析20、60、100kHz 3种超声频率条件下超声参数(超声时间、提取温度、超声能量)对温州蜜柑皮中总酚及抗氧化能力的影响。结果表明:3种超声频率作用下,随着超声时间、提取温度、超声能量的增加,总酚和抗氧化能力呈显著增加趋势,但是总酚和抗氧化能力的最佳超声条件是各不相同的。此外,60kHz的超声频率处理得到温州蜜柑皮提取物总酚和抗氧化能力均高于20、100kHz处理。超声处理后提取物中总酚和抗氧化性呈现出良好的相关性。在20、60、100kHz 3种频率作用下,15℃超声处理后,相关系数R2依次为0.8228、0.8354、0.8435;在30℃时,R2依次为0.7706、0.8735、0.8895;在40℃时,R2依次为0.8626、0.9248、0.8622;在3.2、8、30、56W的超声能量水平下,R2依次为0.9143、0.9958、0.7971,表明在低频超声范围内超声波技术能有效地增强温州蜜柑皮提取物的抗氧化性。

关键词: 温州蜜柑皮, 提取, 总酚, 抗氧化性, 超声频率

Abstract: The effects of ultrasonic-assisted extraction parameters (ultrasonic treatment time, extraction temperature and ultrasonic power) on total phenolic content and antioxidant capacity of methanol extracts from Citrus unshiu peels under different ultrasonic frequencies (20, 60 kHz and 100 kHz) were comparatively analyzed in the present study. The results showed that increasing ultrasonic treatment time, extraction time and ultrasonic power resulted in a notable increase in total phenolic content and antioxidant capacity. However, the optimal extraction conditions for achieving the maximum total phenolic content and antioxidant capacity were different. The extract obtained by 60 kHz ultrasonic-assisted extraction presented higher total phenolic content and antioxidant capacity when compared to those obtained at 20 kHz and 100 kHz. Citrus unshiu peel extracts exhibited an excellent correlation between total phenolic content and antioxidant capacity. The correlation coefficients for the extracts obtained at ultrasonic frequencies of 20, 60 kHz and 100 kHz were 0.8228, 0.8354 and 0.8435 at 15 ℃, 0.7706, 0.8735 and 0.8895 at 30 ℃, 0.8626, 0.9248 and 0.8622 at 40 ℃, respectively. The correlation coefficients for the extracts obtained at ultrasonic powers of 3.2, 8, 30 W and 56 W were 0.9143, 0.9958 and 0.7971, respectively. These findings indicate that ultrasonic treatment at low frequencies can effectively enhance the antioxidant capacity of Citrus unshiu peel extracts.

Key words: Citrus unshiu peel, extraction, total phenolic content, antioxidant capacity, ultrasonic frequency

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