食品科学

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改性番茄皮膳食纤维对糖尿病大鼠肠道菌群的影响

崔 峻1,郑 刚1,胡 娟1,赵国华1,2,*   

  1. 1.西南大学食品科学学院,重庆 400715;2.重庆市农产品加工技术重点实验室,重庆 400715
  • 出版日期:2013-08-15 发布日期:2013-09-03
  • 通讯作者: 赵国华
  • 基金资助:

    国家“863”计划项目(2011AA100805-2)

Impact of Modified Dietary Fiber from Tomato Peel on Intestinal Bacteria in STZ-induced Diabetic Rats

CUI Jun1,ZHENG Gang 1,HU Juan1,ZHAO Guo-hua1,2,*   

  1. 1. College of Food Science, Southwest University, Chongqing 400715, China;
    2. Chongqing Key Laboratory of Agricultural Products Processing, Chongqing 400715, China
  • Online:2013-08-15 Published:2013-09-03
  • Contact: ZHAO Guo-hua

摘要:

目的:研究改性番茄皮渣中膳食纤维对于链尿佐菌素(STZ)诱导糖尿病大鼠肠道菌群的影响。方法:对实验大鼠肠道菌群和粪便中微生物的总DNA进行提取,利用变形梯度凝胶电泳(DGGE)技术对提取总DNA进行电泳,对电泳结果进行比对分析,以确定糖尿病大鼠肠道微生物的组成和变化。结果:肠道菌群研究显示总共有分别来自双歧杆菌属、真杆菌属、梭菌属和乳杆菌属4个属的8株菌为优势菌。其中Bifidobacterium bifidum、Bifidobacterium breve和Eubacterium rectale定殖于肠黏膜,数量保持相对稳定。Lactobacillus murinus在所有的粪便样中均有发现,是粪便中的优势菌种。结论:DGGE分析方法适用于批量处理肠道和粪便样品中的微生物;可溶性番茄皮膳食纤维(SDF)能促进肠道丁酸菌Faecalibacterium prausnitzii的增殖;而超微粉碎番茄皮膳食纤维(UHT)能促进乳酸利用菌Anaerostipes caccae和Eubacterium hallii的增殖。

关键词: 改性番茄皮渣, 膳食纤维, 肠道微生物, 变形梯度凝胶电泳

Abstract:

Objective: To explore the effect of modified dietary fiber from tomato peel on the intestinal flora in STZinduced
diabetic rats. Methods: Microbial total DNA from the intestinal flora and feces of STZ-induced diabetic rats was
extracted. The extracted total DNA was subjected to DGGE electrophoresis to analyze the compositions and variation of the
intestinal flora in diabetic rats. Results: A total of 8 bacterial strains from Bifidobacterium, Eubacterium, Clostridium and
Lactobacillus were detected in the gut and feces of rats. Three bacteria such as Bifidobacterium bifidum, Bifidobacterium
breve, and Eubacterium rectale were colonized in the gut mucosa, and the number of these bacteria remained relatively
stable. Lactobacillus murinus was detected in all fecal samples of rats as the dominant bacterium. Conclusion: DGGE is
suitable for batch analysis of gut and fecal flora. Soluble dietary fiber (SDF) from tomato peel is an important carbon source
for Faecalibacterium prausnitzii. Ultrafine ground dietary fiber from tomato peel is an important carbon source for lactateutilizing
bacteria such as Anaerostipes caccae and Eubacterium hallii.

Key words: modified tomato pomace, dietary fiber, intestinal bacteria, DGGE

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