食品科学

• 工艺技术 • 上一篇    下一篇

碱提酸沉法提取黄秋葵籽蛋白的工艺条件优化

李加兴,向 东,周炎辉,黄 诚   

  1. 1.吉首大学食品科学研究所,湖南 吉首 416000;2.湖南奇异生物科技有限公司,湖南 长沙 410008
  • 出版日期:2013-10-25 发布日期:2013-09-28

Optimization of Alkaline Extraction and Acid Precipitation for the Extraction of Okra Seed Protein

LI Jia-xing,XIANG Dong,ZHOU Yan-hui,HUANG Cheng   

  1. 1. Institute of Food Science, Jishou University, Jishou 416000, China;
    2. Hunan Amazing Grace Biotechnology Co. Ltd., Changsha 410008, China
  • Online:2013-10-25 Published:2013-09-28

摘要:

以黄秋葵籽为原料,采用碱提酸沉法提取其蛋白质,对提取条件进行优化。首先采用单因素试验考察料液比、提取时间、提取温度、pH值等因素对黄秋葵籽蛋白提取率的影响,再以此四因素进行L9(34)正交试验以确定最佳工艺条件。结果表明:碱提黄秋葵籽蛋白的最佳工艺条件为料液比1:30(g/mL)、提取时间60min、提取温度50℃、pH9.0,在此工艺条件下黄秋葵籽蛋白提取率可达72.3%。

关键词: 黄秋葵, 碱提酸沉法, 蛋白质, 正交试验, 工艺条件

Abstract:

The extraction process for okra seed protein by alkaline extraction and acid precipitation method was optimized.
Four process conditions including solid/solvent ratio, extraction time and temperature and solvent pH were investigated for
their effect on the extraction efficiency of okra seed protein and optimized using an L9(34) orthogonal array design. Results
showed that the optimal extraction conditions were solid/solvent ratio of 1:30 (g/mL), extraction time of 60 min, extraction
temperature of 50 ℃, and extraction pH of 9.0. Under these conditions, the yield of okra seed protein was 72.3%.

Key words: okra, alkaline extraction and acid precipitation, protein, orthogonal array design, process conditions