食品科学
• 分析检测 • 上一篇 下一篇
刘 迪,尚 华
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陕西省教育厅专项科研计划项目(12JK0600)
LIU Di, SHANG Hua
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摘要:
基于铜纳米团簇的纳米技术建立简便快速检测牛奶中胆固醇含量的方法。采用铜纳米团簇模拟辣根过氧化物酶催化活性,在胆固醇氧化酶存在条件下,胆固醇被催化氧化产生双氧水,双氧水在铜纳米团簇作用下氧化愈创木酚显色。在优化条件下,比色法检测胆固醇的线性范围为40~320 μg/mL,检出限为5 μg/mL,回收率为99.8%~101.2%。该方法用于牛奶实际样品中检测,精密度、重现性、稳定性和准确性均较好,可作为测定牛奶中胆固醇含量的快速检测方法。
关键词: 铜纳米团簇, 比色检测, 胆固醇, 牛奶
Abstract:
Herein a novel colorimetric method based upon copper nanoclusters for detection of cholesterol in milk was developed. Copper nanoclusters (CuNCs) that possess the catalytic activity of horseradish peroxidase (HRP) which catalyzes the oxidation of colorless guaiacol to amber tetraguaiacol by H2O2, were produced by the reaction of cholesterol and oxygen catalyzed by cholesterol oxidase. Therefore, the oxidation of cholesterol could be transduced into the color change of guaiacol by combining these two reactions. Under the optimum conditions, the absorbance was found to be proportional to the concentrations of cholesterol within the range of 40–320 μg/mL, with a limit of detection (LOD) of 5 μg/mL and recovery rates of 99.8%–101.2%. Moreover, the practicability of this assay in the detection of cholesterol in milk was studied as well, and the results showed good precision, reproducibility, stability and accurateness of this assay method. Thus, this method could be used for quick detection of cholesterol in milk.
Key words: copper nanoclusters, colorimetric detection, cholesterol, milk
中图分类号:
TS207.3
刘 迪,尚 华. 基于铜纳米团簇的纳米探针比色法检测牛奶中胆固醇含量[J]. 食品科学, doi: 10.7506/spkx1002-6630-201412028.
LIU Di, SHANG Hua. Copper Nanoclusters-Based Nanoprobes for Colorimetric Detection of Cholesterol in Milk[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201412028.
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链接本文: https://www.spkx.net.cn/CN/10.7506/spkx1002-6630-201412028
https://www.spkx.net.cn/CN/Y2014/V35/I12/143