食品科学

• 基础研究 • 上一篇    下一篇

紫外光辐照对脱氧雪腐镰刀菌烯醇和T-2毒素的去除作用

邹忠义1,黄 斐1,李洪军2,*   

  1. 1.公安海警学院后勤管理系,浙江 宁波 315801;2.西南大学食品科学学院,重庆 400716
  • 出版日期:2015-10-15 发布日期:2015-10-20

Removal of Deoxynivalenol and T-2 Toxin by Ultraviolet Irradiation

ZOU Zhongyi1, HUANG Fei1, LI Hongjun2,*   

  1. 1. Department of Logistics Management, China Maritime Police Academy, Ningbo 315801, China;
    2. College of Food Science, Southwest University, Chongqing 400716, China
  • Online:2015-10-15 Published:2015-10-20

摘要:

目的:研究紫外光辐照对脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)和T-2毒素的去除作用。方法:测定不同辐照时间、不同辐照距离、不同pH值条件下紫外光辐照对DON和T-2毒素影响,采用高效液相色谱-质谱联用技术测定毒素及其衍生物,外标法定量。结果:经紫外光辐照后,溶液中DON、T-2毒素的质量浓度均随着辐照时间的延长而不断减小,随着辐照距离和pH值的减小而不断减小。pH 7的1.0 μg/mL DON、T-2毒素溶液,在紫外灯功率20 W、辐照距离150 mm条件下辐照60 min后,DON、T-2毒素去除率分别为(84.90±2.52)%、(74.60±2.74)%。紫外光辐照后,毒素溶液中不含有已知的毒素衍生物,可能被转化成新的未知产物。结论:在非碱性条件下,紫外光辐照对DON、T-2毒素具有明显的去除作用。

关键词: 脱氧雪腐镰刀菌烯醇, T-2毒素, 紫外光辐照, 高效液相色谱-质谱

Abstract:

The effects of irradiation time, irradiation distance and pH of toxin solutions on the removal of deoxynivalenol
(DON) and T-2 toxin by ultraviolet (UV) irradiation were investigated. The concentration of toxins and their derivatives
were analyzed by high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and
quantified by an external standard method. The concentration of DON and T-2 toxin in solutions decreased with increasing
irradiation time and decreasing irradiation distance and pH of toxin solutions. The removal rates of DON and T-2 toxin
were (84.90 ± 2.52)% and (74.60 ± 2.74)% after UV irradiation for 60 min under the conditions of 7, 1.0 μg/mL, 20 W and
150 mm for toxin solution pH , toxin concentration , UV light power , and irradiation distance, respectively. The solutions
containing DON and T-2 toxin did not contain known derivatives of both toxins after UV irradiation. DON and T-2 toxin
might be transformed into new unknown products. DON and T-2 toxin were removed obviously by UV irradiation in
non-alkaline condition.

Key words: deoxynivalenol (DON), T-2 toxin, ultraviolet irradiation, high performance liquid chromatography-mass spectrometry

中图分类号: