食品科学

• 安全检测 • 上一篇    下一篇

基于涂膜法FTIR的食用油羰基值快速检测

李易文,陈 倩,张 鹏,李 晶,张 睿,于修烛*   

  1. 西北农林科技大学食品科学与工程学院,陕西 杨凌 712100
  • 出版日期:2016-04-25 发布日期:2016-04-13

Rapid Fourier Transform Infrared Spectroscopic (FTIR) Determination of Carbonyl Value in Edible Oils Using Polyethylene Film

LI Yiwen, CHEN Qian, ZHANG Peng, LI Jing, ZHANG Rui, YU Xiuzhu*   

  1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Online:2016-04-25 Published:2016-04-13

摘要:

为了建立食用油羰基值快速检测方法,以菜籽油为实验材料,茴香醛为羰基标准物配制系列羰基值(0~60 mmol/kg)油样。用涂膜法采集油样光谱,建立羰基值与特征吸收峰1 703/1 570 cm-1处(1 703 cm-1处峰高两点基线均为1 570 cm-1,下同)吸光度的关系。然而,不同种类零羰基值的食用油在特征吸收峰1 703 cm-1处的吸收不相同而引起基底效应。因此,利用二维相关振动光谱技术寻找特征吸收峰的相关吸收峰,以校正食用油间的基底效应。结果表明:油样在1 703 cm-1处的吸光度与羰基值高度线性相关,利用相关吸收峰3 472 cm-1校正后的模型为y=0.005 4x+0.090 9;相关系数(R)为0.991 9,标准偏差(s)为0.013 mmol/kg。模型预测值与国标法测定值高度线性相关,所得方程的斜率接近于1,R值均在0.99以上,两种方法测定结果十分接近。表明涂膜法傅里叶变换红外光谱检测食用油羰基值是可行的。

关键词: 食用油, 傅里叶变换红外光谱, 羰基值, 检测

Abstract:

This study aimed to develop a rapid method for the detection of carbonyl value (CV) in edible oils. Anisaldehyde
was used as carbonyl reference and gravimetrically added to carbonyl-free rapeseed oil. A series of CV (0–60 mmol/kg)
oil samples were determined using polyethylene (PE) film as a spectrum acquisition accessory. A linear relationship between
CV and absorbance at the characteristic absorption peak (1 703/1 570 cm-1, peak at 1 703 cm-1 and two base points at
1 570 cm-1) was established. However, different types of edible oils exhibited substrate effect, which showed that 1 703 cm-1
had variability even among their carbonyl-free samples. To correct the substrate effect, peak absorption at 3 472 cm-1,
as evaluated by the 2-D correlation spectroscopy technique, was used as the relevant absorption peak to 1 703 cm-1.
Subsequently, a liner relationship between both peak heights was established. Results indicated that there was a highly linear
correlation between CV and the absorbance at 1 703 cm-1. The corrected model was y = 0.005 4 x + 0.090 9. with a correlation
coefficient (R) of 0.991 9 and standard deviation of 0.013 mmol/kg. Moreover, there was a good relationship between the values
obtained from the national standard method and the corrected model, with a slope close to 1, and correlation coefficient of more
than 0.99. Thus the PE film-based FTIR method for determination CV in edible oils is workable.

Key words: edible oils, Fourier transform infrared spectroscopy (FTIR), carbonyl value, determination

中图分类号: