食品科学

• 营养卫生 • 上一篇    下一篇

基于HepG2细胞模型研究普洱茶茶色素的抗氧化作用

陈亚蓝1,王雪青1,*,王怡雯1,郑子晴1,叶美霞1,付芳芳1,宋文军1,王素英1,白晓丽2,李长文2   

  1. 1.天津商业大学生物技术与食品科学学院,天津市食品与生物技术重点实验室,天津 300134;
    2.云南天士力帝泊洱生物茶集团有限公司,云南 普洱 665100
  • 出版日期:2017-01-15 发布日期:2017-01-16

Antioxidative Capacity of Pigments from Pu-erh Tea Based on HepG2 Cell Model

CHEN Yalan1, WANG Xueqing1,*, WANG Yiwen1, ZHENG Ziqing1, YE Meixia1, FU Fangfang1, SONG Wenjun1, WANG Suying1, BAI Xiaoli2, LI Changwen2   

  1. 1. Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; 2. Yunnan Tasly Deepure Biological Tea Group Co. Ltd., Pu Erh 665100, China
  • Online:2017-01-15 Published:2017-01-16

摘要: 采用正常培养和油酸诱导培养的HepG2细胞为模型,通过测定普洱茶茶色素对HepG2细胞外谷胱甘肽(glutathione,GSH)含量,过氧化氢酶(catalase,CAT)活力和细胞内丙二醛(malondialdehyde,MDA)含量的影响,以研究普洱茶茶色素的抗氧化作用。结果显示,普洱茶茶色素对正常培养的HepG2细胞的抗氧化作用影响不显著,而对油酸诱导的HepG2细胞模型抗氧化作用有显著的提高。抗氧化作用提高的程度依赖于普洱茶茶色素的质量浓度。当400 μg/mL普洱茶茶色素作用HepG2油酸诱导模型24 h,细胞外GSH含量由0.004 4 g/L增加到0.010 3g/L,CAT活力由0.136 U/mL提高至1.174 U/mL,细胞内MDA含量由15.146 nmol/mg减少到7.635 nmol/mg,从而使这些指标接近正常培养HepG2细胞模型水平。因此,普洱茶茶色素的抗氧化作用是通过提高清除活性氧的酶活力和促进合成还原性物质来干预细胞的氧化应激。

关键词: HepG2, 抗氧化, 细胞模型, 普洱茶, 茶色素

Abstract: In terms of catalase (CAT) activity and malondialdehyde (MDA) and glutathione (GSH) levels, the antioxidative
capacity of pigments from Pu-erh tea was assessed using HepG2 cell models cultured with normal or oleic acid enriched
medium. The results showed that the antioxidative capacity of Pu-erh tea pigments was significant on the oleic acid-induced
HepG2 cell model in a concentration-dependent manner rather than on the normally cultured ones. The extracellular GSH
content and CAT activity in HepG2 cells induced by oleic acid for 24 h with added Pu-erh tea pigments increased from 0.004 4
to 0.010 3 g/L and from 0.136 to 1.174 U/mL, respectively, and MDA content reduced from 15.146 to 7.635 nmol/mg, almost
reaching the normal values. Therefore, the antioxidative mechanism of Pu-erh tea pigments may be due to enhancing reactive
oxygen species (ROS) scavenging enzyme activities, promoting the synthesis of reducing substances and therefore altering
the status of oxidative stress of HepG2 cells.

Key words: HepG2, antioxidant capacity, cell model, Pu-erh tea, tea pigments

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