食品科学 ›› 2019, Vol. 40 ›› Issue (1): 155-161.doi: 10.7506/spkx1002-6630-20170922-328

• 营养卫生 • 上一篇    下一篇

低值海洋鱼低聚肽抗氧化和抗疲劳活性

丁树慧1,2,齐曼婷3,齐 斌2,*,张 晶1,*   

  1. 1.吉林农业大学中药材学院,吉林 长春 130118;2.常熟理工学院生物与食品工程学院,苏州市食品生物技术重点实验室,发酵工程技术研究中心,江苏 常熟 215500;3.谱尼测试集团江苏有限公司,江苏 苏州 215000
  • 出版日期:2019-01-15 发布日期:2019-01-22
  • 基金资助:
    江苏省科技厅重点研发计划项目(BE2017316;BE2017326);国家高技术研究发展计划(863计划)项目(2013AA102203-03)

Antioxidant and Anti-fatigue Activity of Marine Trash Fish-Derived Oligopeptide

DING Shuhui1,2, QI Manting3, QI Bin2,*, ZHANG Jing1,*   

  1. 1. College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China; 2. Key Laboratory of Food and Biotechnology of Suzhou, Fermentation Engineering Technology Research Center, College of Biological and Food Engineering, Changshu Institute of Technology, Changshu 215500, China; 3. Pony Testing International Group, Suzhou 215000, China
  • Online:2019-01-15 Published:2019-01-22

摘要: 目的:研究低值海洋鱼(以鳀鱼为主的海产小杂鱼)低聚肽体外抗氧化活性和抗疲劳作用。方法:通过1,1-二苯基-2-三硝基苯肼等4 种体外化学模型、DNA氧化损伤模型及小鼠生理生化指标综合评价低值海洋鱼低聚肽的抗氧化与抗疲劳能力。结果表明:低聚肽清除1,1-二苯基-2-三硝基苯肼自由基、羟自由基和超氧阴离子自由基半抑制浓度(half maximal inhibitory concentration,IC50)分别为(4.76±0.57)、(5.09±0.27)、(5.91±0.23)mg/mL,还原力IC50为(1.31±0.12)mg/mL;与空白组相比,不同剂量的低值海洋鱼低聚肽均延长了小鼠力竭游泳时间,降低了小鼠疲劳后乳酸和尿素氮含量,提高了肝糖原含量和超氧化物歧化酶、过氧化氢酶及谷胱甘肽过氧化物酶活力。结论:低值海洋鱼低聚肽具有一定的抗氧化活性,并且能够提高运动耐力,缓解疲劳。

关键词: 低值鱼低聚肽, 抗氧化, 抗疲劳, 自由基

Abstract: Objective: To investigate the in vitro antioxidant activity and anti-fatigue activity of oligopeptide derived from defatted fish meal from marine trash fish (mainly anchovy). Methods: The in vitro antioxidant activity was evaluated using four chemical model systems and DNA oxidative damage model system and the anti-fatigue activity was assessed by measuring physiological and biochemical indexes of mice. Results: The oligopeptide scavenged 1,1-diphenyl-2- picrylhydrazyl (IC50 = (4.76 ± 0.57) mg/mL), hydroxyl (IC50 = (5.09 ± 0.27) mg/mL) and superoxide anion radicals (IC50 = (5.91 ± 0.23) mg/mL) and had reducing power (IC50 = (1.31 ± 0.12) mg/mL). Compared with the control group, the oligopeptide at all dosages could significantly increase exhaustive swimming time, decrease the levels of lactic acid and blood urea nitrogen, and increase liver glycogen content and the activities of endogenous cellular antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in mice after exhaustive swimming exercise. Conclusion: The oligopeptide derived from marine trash fish has antioxidant capacity and can increase endurance capacity and facilitate recovery from physical exhaustion.

Key words: marine trash fish-derived oligopeptide, antioxidant, anti-fatigue, free radical

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