关键词: 杜仲, 苯丙素类, 高效液相色谱, 不同生长年限, 不同部位

Abstract: Objective: To establish a high performance liquid chromatography (HPLC) method for the determination of five phenylpropanoids in Eucommia ulmoides Oliv. from different ages and parts. Methods: The samples were separated on HiQ Sil C18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase consisting of 0.2% formic acid (A) and acetonitrile (B) with gradient elution (4%–12% B between 0 and 12 min; 12%–14% B between 12 and 35 min) at a flow rate of 1.0 mL/min and detected at 277 nm, and the column temperature was kept at 25 ℃. The injection volume was 5 μL. Results: The method had good linearity in the ranges of 0.04–1 mg/mL (R2 = 0.999 7) for chlorogenic acid, 0.001 6–0.029 mg/mL (R2 = 0.994 0) for caffeic acid, 0.001 6–0.04 mg/mL (R2 = 0.998 9) for protocatechuic acid, 0.008–0.04 mg/mL (R2 = 0.998 7) for coniferin, and 0.013–0.029 mg/mL (R2 = 0.998 6) for syringoside. The average recoveries of spiked samples ranged from 94.05%–107.02%, with relative standard deviations (RSDs) in the range of 0.95%–4.11%. Conclusion: This method met the requirements of methodological validation, and it could be used for the determination of chlorogenic acid, caffeic acid, protocatechuic acid, coniferin, and syringoside in different parts of E. ulmoides Oliv. The total content of five phenylpropanoid compounds in leaves was higher than in barks, suggesting that E. ulmoides Oliv. leaves can be considered an alternative to its barks in functional foods.

Key words: Eucommia ulmoides Oliv., phenylpropanoid, high performance liquid chromatography (HPLC), different ages, different parts