紫花芸豆肽修复H2O2对HepG2细胞的氧化应激损伤

doi:10.7506/spkx1002-6630-20181103-030

食品科学 ›› 2020, Vol. 41 ›› Issue (1): 175-182.doi: 10.7506/spkx1002-6630-20181103-030

紫花芸豆肽修复H2O2对HepG2细胞的氧化应激损伤

马萍，程天赋，郭增旺，周义，王欣，曹冬梅

（黑龙江八一农垦大学食品学院，黑龙江大庆 163319）

出版日期:2020-01-15 发布日期:2020-01-19
基金资助:
黑龙江省科技厅应用技术研究与开发计划项目（GZ13B003）；黑龙江省教育厅科学技术研究项目（12541586）

Cytoprotective Effect of Phaseolus vulgaris Peptides against H2O2-Induced Oxidative Damage in HepG2 Cells

MA Ping, CHENG Tianfu, GUO Zengwang, ZHOU Yi, WANG Xin, CAO Dongmei

(College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China)

Online:2020-01-15 Published:2020-01-19

摘要/Abstract

摘要： 目的：研究紫花芸豆肽（Phaseolus vulgaris peptides，PVPs）对H2O2诱导的HepG2细胞氧化损伤的保护作用及其作用机制。方法：体外培养HepG2细胞，实验分为空白组、模型组（正常培养24?h后加2?mmol/L?H2O2刺激1?h）以及PVPs低、中、高剂量组（分别用50、100、200?μg/mL PVPs处理24?h后，加2?mmol/L?H2O2刺激1?h），采用水溶性四唑盐法（WST-1法）检测细胞增殖率，流式细胞仪检测胞内活性氧（reactive oxygen species，ROS）水平，酶联免疫吸附测定法检测胞内抗氧化物酶系活力，Western blot法检测凋亡蛋白表达量。结果：PVPs能缓解H2O2导致的HepG2细胞生长抑制，降低胞内ROS、丙二醛水平，提高超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活力，并且下调p53、Caspase-3蛋白表达量。结论：PVPs对H2O2引起的HepG2细胞氧化损伤具有一定的保护作用，能抑制凋亡蛋白的表达，同时可以调节细胞的氧化还原系统、清除胞内ROS、提高胞内抗氧化物酶系的活力。

关键词: 紫花芸豆肽, 氧化应激, 抗氧化物酶系, 凋亡蛋白

Abstract: The purpose of this experiment was to investigate the protective effect of Phaseolus vulgaris peptides (PVPs) on H2O2-induced oxidative damage in HepG2 cells and to elucidate the underlying mechanism. HepG2 cells were cultured in vitro, which were divided into blank group, model group (subjected to 2 mmol/L H2O2 stimulation for 1 h after 24 h culture under normal conditions), and low-, medium- and high-dose PVPs (subjected to 2 mmol/L H2O2 stimulation for 1 h after 24 h culture in the presence of PVPs at 50, 100 and 200 μg/mL) groups. The cell proliferation rate was measured by WST-1 assay, the reactive oxygen species (ROS) level by flow cytometry, and the intracellular antioxidant enzyme activity by enzyme linked immunosorbent assay, and the expression of apoptotic proteins by Western blot. The results showed that PVPs could alleviate growth inhibition induced by H2O2 in HepG2, reduce the levels of ROS and intracellular malondialdehyde, increase superoxide dismutase, catalase and glutathione peroxidase activity, and down-regulate p53 and caspase-3 protein expression. In conclusion, PVPs has a protective effect on H2O2-induced oxidative damage in HepG2 cells through inhibiting the expression of apoptotic proteins, regulating the cellular redox system, scavenging intracellular reactive oxygen species, and increasing intracellular activity of antioxidant enzymes.

Key words: Phaseolus vulgaris peptides, oxidative stress, antioxidant enzyme system, apoptosis protein

中图分类号:

马萍，程天赋，郭增旺，周义，王欣，曹冬梅. 紫花芸豆肽修复H2O2对HepG2细胞的氧化应激损伤[J]. 食品科学, 2020, 41(1): 175-182.

MA Ping, CHENG Tianfu, GUO Zengwang, ZHOU Yi, WANG Xin, CAO Dongmei. Cytoprotective Effect of Phaseolus vulgaris Peptides against H2O2-Induced Oxidative Damage in HepG2 Cells[J]. FOOD SCIENCE, 2020, 41(1): 175-182.

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紫花芸豆肽修复H2O2对HepG2细胞的氧化应激损伤

Cytoprotective Effect of Phaseolus vulgaris Peptides against H2O2-Induced Oxidative Damage in HepG2 Cells

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