食品科学 ›› 2018, Vol. 39 ›› Issue (12): 239-246.doi: 10.7506/spkx1002-6630-201812037

• 工艺技术 • 上一篇    下一篇

黑果腺肋花楸花色苷提取工艺优化及其抗氧化活性和组成鉴定

位路路,林杨,王月华,孟宪军*   

  1. (沈阳农业大学食品学院,辽宁?沈阳 110866)
  • 出版日期:2018-06-25 发布日期:2018-06-15
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2017YFD0400704-4)

Optimization of Extraction of Anthocyanins from Berries of Aronia melanocarpa and Their Antioxidant Activity and Composition

WEI Lulu, LIN Yang, WANG Yuehua, MENG Xianjun*   

  1. (College of Food Science, Shenyang Agricultural University, Shenyang 110866, China)
  • Online:2018-06-25 Published:2018-06-15

摘要: 目的:优化超声波辅助提取黑果腺肋花楸花色苷的条件,测定花色苷的抗氧化能力,鉴定黑果腺肋花楸花色苷提取物的组成成分。方法:采用响应面法优化花色苷提取条件,通过1,1-二苯基-2-三硝基苯肼自由基清除能力、2,2’-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基清除能力及Fe3+还原能力方法评价其抗氧化能力,高效液相色谱-质谱联用法进行成分分析。结果表明:提取温度60?℃、超声功率100?W、料液比1∶30(g/mL)、超声时间31?min、乙醇体积分数64%和pH?2.0时为最优提取条件,此时黑果腺肋花楸花色苷含量可达(3.61±0.01)mg/g。体外抗氧化实验表明,在相同质量浓度条件下,黑果腺肋花楸花色苷提取物的抗氧化活性明显高于VC。组分鉴定表明黑果腺肋花楸花色苷提取物中共有6?种花色苷,其中矢车菊-己糖苷二聚体和矢车菊-3,5-二己糖苷为新检测出的2?种花色苷。

关键词: 黑果腺肋花楸, 花色苷, 响应面, 抗氧化, 高效液相色谱-质谱联用

Abstract: This study aimed to optimize the ultrasonic-assisted extraction of anthocyanins from Aronia melanocarpa and to determine the antioxidant capacity and constituents of the obtained extract. The optimization was carried out by response surface methodology. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) radical (ABTS+·) scavenging capacity and Fe3+ reducing ability of plasma (FRAP) assays were used to evaluate the antioxidant capacity. The anthocyanin components were analyzed by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The results showed that the optimum extraction conditions were as follows: extraction temperature 60 ℃, ultrasonic power 100 W, solid-to-liquid 1:30 (g/mL), ultrasonic time 31 min, ethanol concentration 64%, and pH 2.0. Under these conditions, the content of anthocyanins was determined to be (3.61 ± 0.01) mg/g. The in vitro antioxidant activity of the anthocyanin extract was significantly higher than that of vitamin C at the same concentration. Six anthocyanins including two new compounds, dimer of cyanidin-3-hexoside and cyanidin-3,5-dihexoside, were detected in the extract.

Key words: Aronia melanocarpa, anthocyanins, response surface methodology, antioxidant activity, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)

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