• 安全检测 •

### 基于核酸适配体的比色传感策略用于牛奶中沙门氏菌的快速检测

1. （1.上海海洋大学食品学院，上海 201306；2.上海水产品加工及贮藏工程技术研究中心，农业农村部水产品贮藏保鲜质量安全风险评估实验室（上海），上海 201306）
• 发布日期:2022-09-28
• 基金资助:
国家自然科学基金面上项目（21775102）；上海市自然科学基金项目（20ZR1424100）

### Rapid Visual Detection of Salmonella typhimurium Using a Colormetric Aptamer Sensor Based on Gold Nanoparticles

ZHANG Qianwen, TAO Qing, ZHAO Tingting, ZHANG Tong, YAN Juan

1. (1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2. Shanghai Engineering Research Center of Aquatic-product Processing & Preservation, Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai 201306, China)
• Published:2022-09-28

Abstract: An aptamer sensor (aptasensor) based on gold nanoparticles (AuNPs) was developed for the rapid visual detection of Salmonella typhimurium in foods. The aptamer specifically bound to S. typhimurium in samples to be analyzed, and a capturing probe that could adsorb onto the surface of AuNPs was released, avoiding subsequent aggregation of AuNPs induced by salts. In the absence of S. typhimurium, salts induced the aggregation of AuNPs, leading to color changes visible to the naked eye in the solution, thus allowing for the rapid and simple detection of S. typhimurium. The feasibility, detection performance, and specificity of the developed aptasensor were characterized. The aptasensor had a good response performance to S. typhimurium in the range of 10–109 CFU/mL. A linear regression model equation was fitted as follows: y = ?0.129 98x + 1.244 11 (R2 = 0.992 62), and the detection limit was as low as 124 CFU/mL. Four kinds of non-target bacteria were detected by this aptasensor with good specificity. Because of its high sensitivity, simple operation, rapidity and low cost, it holds great promise for rapid and in situ food safety detection.