食品科学 ›› 2022, Vol. 43 ›› Issue (7): 254-262.doi: 10.7506/spkx1002-6630-20210325-312

• 专题论述 • 上一篇    下一篇

基于噬菌体的电化学生物传感器在检测食源性病原菌中的研究进展

王吉,李慧慧,朱文娟,王佳,王小红   

  1. (1.华中农业大学食品科学技术学院,湖北 武汉 430070;2.华中农业大学理学院,湖北 武汉 430070)
  • 出版日期:2022-04-15 发布日期:2022-04-26
  • 基金资助:
    国家自然科学基金面上项目(31772054);“十三五”国家重点研发计划重点专项(2017YFC1600100); 河南省重点研发与推广专项(202102110058)

Progress in the Development and Application of Bacteriophage-Based Electrochemical Biosensors for Detection of Foodborne Pathogenic Bacteria

WANG Ji, LI Huihui, ZHU Wenjuan, WANG Jia, WANG Xiaohong   

  1. (1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;2. College of Science, Huazhong Agricultural University, Wuhan 430070, China)
  • Online:2022-04-15 Published:2022-04-26

摘要: 食源性病原菌是引发食源性疾病暴发的主要原因之一,给人类的生命健康造成极大的威胁,因此其快速准确检测一直备受关注。近年来,电化学生物传感器作为一种快速、灵敏、简便的检测方法已经得到广泛的研究,其研究内容集中于识别元件的开发和固定以及样品的检测应用。噬菌体是一种能特异性侵染细菌的病毒,可将其作为电化学生物传感器中重要的识别元件来开发食源性病原菌的检测方法。与酶、抗体、核酸等识别元件相比,噬菌体更加容易获得,对环境的耐受性强、性质稳定。同时基于噬菌体的阻抗型、安培型等电化学技术因其灵敏度高逐步得到重视和应用。因此,本文着重对噬菌体应用于电化学生物传感器中的固定方法和应用现状进行综述,并对其未来的发展趋势进行展望。

关键词: 食源性病原菌;噬菌体;电化学生物传感器;固定方法

Abstract: Foodborne pathogens, a major cause of foodborne disease outbreaks, are a great threat to human life and health. In this context, rapid and accurate detection of foodborne pathogens is of great interest. In recent years, electrochemical biosensors as a fast, sensitive and simple detection method have been widely studied, and most studies focus on the development and immobilization of recognition elements and the application of electrochemical biosensors in sample detection. Phages, bacterial viruses specifically infect their hosts, can be used as an important recognition element in electrochemical biosensor for the detection of foodborne pathogens. Compared with enzyme, antibody, nucleic acid and other recognition elements, phages are easier to obtain, more tolerant to the environment, and more stable. Simultaneous phage-based electrochemical techniques with impedance and amperometric probes have gained increasing attention and been applied due to their high sensitivity. In this paper, the phage immobilization methods and the current status of the application of phages in electrochemical biosensors are described, and future trends are also discussed.

Key words: foodborne pathogens; bacteriophage; electrochemical biosensor; immobilization method

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