食品科学 ›› 2023, Vol. 44 ›› Issue (18): 40-48.doi: 10.7506/spkx1002-6630-20220818-222

• 食品化学 • 上一篇    

玉米醇溶蛋白负载植物甾醇纳米颗粒的制备及性能

杨婷婷,任李顺,陈光未,黄艾祥   

  1. (云南农业大学食品科学技术学院,云南 昆明 650201)
  • 发布日期:2023-09-29
  • 基金资助:
    云南省重大科技专项计划项目(202102AE090027-1);云南农业大学第十五届学生创新创业行动基金项目(2022ZKY255); 云岭产业技术领军人才项目(云发改人事(2014)1782号)

Preparation and Properties of Zein Loaded Phytosterol Nanoparticles

YANG Tingting, REN Lishun, CHEN Guangwei, HUANG Aixiang   

  1. (College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China)
  • Published:2023-09-29

摘要: 为提高植物甾醇(phytosterols,PS)的稳定性、水溶性和胃肠缓释能力,采用反溶剂法制备玉米醇溶蛋白-植物甾醇(zein-phytosterols,Zein-PS)纳米颗粒,以纳米粒的稳定性和包埋率为指标,通过单因素和正交试验确定最佳制备工艺条件,采用扫描电子显微镜(scanning electron microscopy,SEM)、傅里叶变换红外光谱(Fourier transform infrared spectrometry,FTIR)及胃肠模拟消化并研究其结构和功能的特性。结果表明:Zein-PS纳米粒最佳工艺为Zein与PS质量比为15∶1、水合时间2 h、水合温度55 ℃、超声时间20 min,所得纳米粒的包封率为84.97%,粒径为(479.76±0.38)nm,Zeta电位为(-22.79±0.015)mV,粒径较小且稳定性高。当质量比为15∶1时,纳米颗粒的复溶性和水溶解度较好。SEM揭示纳米粒表面形成致密的网络结构及FTIR显示其在3 313.16 cm-1和1 523.51 cm-1处发生了变化,这说明二者存在氢键、静电相互作用。与PS相比,纳米粒在胃肠液中释放率分别减少49.03%和28.11%,具有较好的缓释效果。此外,纳米粒在4 ℃和25 ℃贮藏30 d后,4 ℃粒径变化较小,包埋率仍能保持在70%以上。因此,Zein-PS复合纳米颗粒具有良好的稳定性和缓释性能,在食品工业中具有潜在的应用前景。

关键词: 玉米醇溶蛋白;植物甾醇;纳米粒;缓释性能

Abstract: This study was undertaken in order to improve the stability, water solubility and gastrointestinal release of phytosterols (PS). Zein-PS nanoparticles were prepared by the anti-solvent method. Single factor experiments and orthogonal array design methods were adopted to optimize the preparation conditions based on the stability and encapsulation efficiency of zein-PS nanoparticles. The structure and functional properties of the prepared samples were characterized by using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and simulated gastrointestinal digestion. The results showed that the optimal preparation conditions were determined as follows: zein/PS ratio of 15:1 (m/m), hydration time of 2 h, hydration temperature of 55 ℃, and ultrasonic treatment time of 20 min. The encapsulation efficiency of the nanoparticles prepared using the optimal conditions was 84.97%, particle size (479.76 ± 0.38) nm, and zeta potential (−22.79 ± 0.015) mV, suggesting small particle size and high stability. The nanoparticles with a zein/PS ratio of 15:1 had better re-solubility and water solubility. SEM revealed the formation of a compact network structure on the surface of the nanoparticles and FTIR spectra showed that it changed at 3 313.16 and 1 523.51 cm-1, indicating the presence of hydrogen bonding and electrostatic interaction between zein and PS. Compared with PS, the release rates of the nanoparticles in simulated gastrointestinal fluids were reduced by 49.03% and 28.11%, respectively, showing a good sustained-release effect. In addition, after storage for 30 days, the change in the particle size of the nanoparticles was smaller at 4 ℃ than at 25 ℃, and the encapsulation efficiency remained above 70%. Therefore, zein-PS composite nanoparticles have good stability and sustained release property, which has potential application prospects in the food industry.

Key words: zein; phytosterol; nanoparticles; sustained release property

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