食品科学 ›› 2000, Vol. 21 ›› Issue (12): 13-15.

• 基础研究 • 上一篇    下一篇

酶法改性玉米蛋白功能特性的研究

 鲁晓翔, 陈新华, 唐津忠   

  1. 天津商学院食品科学系
  • 出版日期:2000-12-15 发布日期:2011-12-05

Studies on the Functional Property of Enzymatic Modified Corn Protein

 LU  Xiao-Xiang, CHEN  Xin-Hua, TANG  Jin-Zhong   

  • Online:2000-12-15 Published:2011-12-05

摘要: 利用胰蛋白酶对玉米蛋白进行水解,得到了不同水解度的玉米蛋白水解液,并对酶解蛋白液的溶解性、起泡性和乳化性等功能特性进行了测定。结果表明,对原料进行加热、加碱或添加0.1%的亚硫酸钠的预处理,可以大大提高酶解的效果,水解度可由7.1%分别提高到17.2%、17.6%和23.6%;酶改性可显著提高玉米蛋白的水溶性、起泡性和乳化性。在一定的水解度范围内,这些功能特性随着水解度的增大而增大,但继续增加水解度,起泡性和乳化性均有下降的趋势。当底物浓度为5%、酶与底物比6000 u/g、pH=9.0、温度为500C、水解240min,水解度达18.6%,玉米蛋白有较好的起泡性和乳化性,分别为85%和7.8%。

关键词: 玉米蛋白, 胰蛋白酶, 功能特性

Abstract: In this paper, the protein of Corn Gluten Meal was hydrolyzed by trypsin, where corn protein hydrolysate with different degrees of hydrolysis (DH) was obtained. The effects of hydrolysis on the functional properties, such as water solubility, foam capacity and emulsifying capacity were investigated. The results showed that the material pre-treated with appropriate heating, alkaline or addition of 0.1% Na2SO3 gave better hydrolysis results, DH was raised from 7.1% to 17.2%, 17.6% and 23.6% respectively. The results also showed that enzymatic hydrolysis could increase the water solubility, foam capceity and emulsifying capacity of the hydrolysates strongly. Within some range of hydrolysis, these functional properties increased with the increase of DH, but further increasing the degree of hydrolysis, the foam and the emulsifying capacity would contrarily decrease. When the corn protein concentration was 5%, enzyme and protein ratio 6000u/g, pH=9.0, temperature 500C and hydrolysis time 240 minutes and degree of hydrolysis value reaching 18.6%, the better functional property was obtained, 85% for foam capacity and 7.8 for emulsifying capacity.

Key words: Corn protein Trypsin Functional property