黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究

食品科学 ›› 2004, Vol. 25 ›› Issue (2): 167-170.

黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究

陈留勇, 孟宪军, 贾薇, 孔秋莲, 郑玉生, 章丽丽

沈阳农业大学食品学院，上海农业科学院食用菌所，上海市农业科学院农产品保鲜加工中心

出版日期:2004-02-15 发布日期:2011-10-24

The Study on The Antitumor Activity and Scavenging Free Radical and Immune Effect of The Water-soluble Polysaccharides from A.Persica.L.var.seleropersica

CHEN Liu-Yong, MENG Xian-Jun, JIA Wei, KONG Qiu-Lian, ZHENG Yu-Sheng, ZHANG Li-Li

1.Food College of Shen Yang Agriculture University;2.Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences;3.Shanghai Academy of Pharmaceuti-cal Industry

Online:2004-02-15 Published:2011-10-24

摘要/Abstract

摘要： 从黄桃果肉中提取出两种水溶性多糖HTP1和HTP2,本文研究了HTP1和HTP2对自由基的清除作用,以及以小鼠为动物模型研究了它们在抗肿瘤及提高免疫力方面的功效,实验结果表明,HTP1和HTP2对羟基自由基(•OH)均有显著的清除作用,其IC50分别为270.92μg/ml和4.361mg/ml。HTP1对超氧阴离子自由基(O2)有明显清除作用,其IC50约为401.3μg/ml,而HTP2无清除超氧阴离子自由基(O2)的能力。HTP1及HTP2各分别为100mg/kg、200mg/kg剂量ip×10qd方案,对小鼠Lewis肺癌足趾皮下接种的抑瘤率分别为54.69%、43.44%及47.08%、44.48%。对荷Lewis肺癌小鼠的NK及淋巴细胞转化的活性,HTP1和HTP2两个剂量组成均有较明显的促进和提高。

关键词: 黄桃, 多糖, 清除自由基, 抗肿瘤, 提高免疫力

Abstract: The water soluble polysaccharides of peach were extracted in water, and then isolated by ethanol, through DEAE-sehparose cation exchange column chromatography and Sephacryl G-300 gel column chromatography, we got two polysaccha-rides HTP1 and HTP2. In this paper, we studied the Effect of Scavenging Free-radical of HTP1 and HTP2. The results showedthat they had a evident effect in scavenging •OH, Their IC50 were 270.92μg/ml and 4.361mg/ml. HTP1 had a evident effectin scavenging O2-, the IC50 was 401.3μg/ml, but HTP2 had no effect on O2-. HTP1 and HTP2 had relatively high growth-inhibition activities against Lewis lung carcinoma and enhanced immune function of the mice, whice took it. HTP1 and HTP2were injected by C5BL/6 mice at two dosages of 100 mg/kg per day and 200 mg/kg per day, the inhibition rate of Lewis lungcarcinoma by subcutaneous inoculation to mice at toe were about 43.44%～54.69% and 44.48%～47.08% respectively.Meanwhile, HTP1 and HTP2 enhanced the activities of NK cells and lymphocyte activities of tumor-bearing mice.

Key words: A.Persica.L.var.seleropersica, polysaccharides, antitumor activity, scavenging free-radical Enhance immune

陈留勇, 孟宪军, 贾薇, 孔秋莲, 郑玉生, 章丽丽. 黄桃水溶性多糖的抗肿瘤作用及清除自由基、提高免疫活性研究[J]. 食品科学, 2004, 25(2): 167-170.

CHEN Liu-Yong, MENG Xian-Jun, JIA Wei, KONG Qiu-Lian, ZHENG Yu-Sheng, ZHANG Li-Li. The Study on The Antitumor Activity and Scavenging Free Radical and Immune Effect of The Water-soluble Polysaccharides from A.Persica.L.var.seleropersica[J]. FOOD SCIENCE, 2004, 25(2): 167-170.

[1]	孙聪，郑明珠，许秀颖，蔡丹，刘美宏，曹勇，刘景圣. 玉木耳多糖对杂粮粥糊化、流变及老化特性的影响[J]. 食品科学, 2021, 42(8): 46-51.
[2]	项婷，夏琛，刘健华，王超然，沈建福. 蛹虫草中新化合物的分离纯化、结构鉴定及抗肿瘤活性[J]. 食品科学, 2021, 42(8): 235-242.
[3]	詹慧，宋田源，余刚，姜泽东，杜希萍，朱艳冰，倪辉，李清彪. 基于酶活力模型和细胞模型分析红毛藻多糖降血脂活性[J]. 食品科学, 2021, 42(7): 142-148.
[4]	王丽波，高婧宇，李腾飞，李莲玉，刘博，张洪龑，杨昱，徐雅琴. 硒化蒲公英多糖的制备、结构表征及益生菌促增殖活性[J]. 食品科学, 2021, 42(7): 169-175.
[5]	石璞洁，许诗琦，王震宇，吴超，卢卫红，杜明. 乳铁蛋白生物活性肽及其功能机制研究进展[J]. 食品科学, 2021, 42(7): 267-274.
[6]	耿超，卫莹，沈明花. 榆干离褶伞溶栓酶对脂多糖诱导的血管内皮细胞损伤的保护作用[J]. 食品科学, 2021, 42(5): 129-136.
[7]	秦丹丹，吴琼，白洋，曹慧馨，刘梦林. 黑木耳多糖/壳聚糖可食性复合膜的制备及对鲜牛肉的保鲜效果[J]. 食品科学, 2021, 42(5): 246-251.
[8]	谢文，陈华国，赵超，龚小见，周欣. 枸杞多糖的生物活性及作用机制研究进展[J]. 食品科学, 2021, 42(5): 349-359.
[9]	魏晨业，包晓玮，王娟，何梦梦，曾兰君，张亚涛. 沙棘多糖分离纯化及抗氧化活性[J]. 食品科学, 2021, 42(4): 227-232.
[10]	李妍，崔维建，赵城彬，吴玉柱，王思琪，曹勇，许秀颖，刘景圣. 玉米淀粉-玉木耳多糖复配体系理化及结构性质[J]. 食品科学, 2021, 42(4): 58-64.
[11]	姜慧，孔立敏，王翀，王彦波，傅玲琳，周涛. 条斑紫菜多糖的降解条件优化及其生物活性[J]. 食品科学, 2021, 42(3): 38-47.
[12]	茅芝娟，张昆明，黄永春，郭艳，杨锋，任仙娥，艾硕. 壳聚糖W/O型乳状液的超声辅助制备及其稳定性[J]. 食品科学, 2021, 42(2): 8-16.
[13]	马永强，韩烨，张凯，王鑫，王峙力. 甜玉米芯多糖对胰岛素抵抗HepG2细胞糖代谢功能的影响[J]. 食品科学, 2021, 42(17): 170-176.
[14]	汪少芸，冯雅梅，伍久林，陈旭，冯佳雯，施晓丹，蔡茜茜，张芳. 蛋白质-多糖多尺度复合物结构的形成机制及其应用前景[J]. 食品科学, 2021, 42(17): 1-9.
[15]	何萍萍，郑雅君，郑明静，姜泽东，杜希萍，朱艳冰，倪辉，李清彪. 红毛藻多糖对H2O2诱导的Caco-2细胞氧化损伤的保护作用[J]. 食品科学, 2021, 42(17): 113-120.