食品科学 ›› 2004, Vol. 25 ›› Issue (7): 117-120.

• 工艺技术 • 上一篇    下一篇

谷氨酰胺(Gln)活性肽营养液的制备研究

 任国谱, 瞿伟菁   

  1. 湖南亚华乳业博士后工作站,华东师范大学生命科学学院
  • 出版日期:2004-07-15 发布日期:2011-10-24

Preparation of Bioactive Glutamine-enriched Oligopeptide Nutrient

 REN  Guo-Pu, QU  Wei-Jing   

  1. 1.Hunan Yahua Post-docterate Station;2.College of Life Science,East ChinaNormal University
  • Online:2004-07-15 Published:2011-10-24

摘要: 50L的反应体积,采用复合酶A和碱性蛋白酶两次酶解玉米蛋白制备Gln活性肽,复合酶A的选用条件为pH8.5、底物浓度[S]2%、酶用量[E]9万U/100g玉米蛋白、温度40℃,水解度DH10;碱性蛋白酶的条件为pH8.5、[E]3万U/100g玉米蛋白、温度45℃,水解1h。酶解液通过超滤(COMW5000)、反渗浓缩(10kg/cm2,常温2h),最后产品中的有效Gln组成(有效Gln占总氨基酸的百分率)为17.9%,总氮得率33.3%。

关键词: 玉米蛋白, 谷氨酰胺, 活性肽, 营养液, 制备

Abstract: Glutamine-enriched oligopeptide nutrient was obtained by hydrolyzing corn protein with prozyme A and alcalasesuccessively in the 50 liter volume. The conditions for prozyme A were: pH8.5, [s] 2%, [E] 90000 units per 100 grams corn protein,DH10 and temperature 40 ℃. The conditions for alcalase were: pH8.5, [E] 30000 units per 100 grams corn protein, 1h andtemperature 45℃. The hydrolysate was separated by ultrafilter (COMW 5000), and then condensed by reverse osmosis (10kg/cm2, room temperature, 2h). The ratio of effective glutamine to total amino acids was 17.9%. The protein yield was 33.3%.

Key words: corn protein, glutamine, bioactive oligopeptide, nutrition, preparation