食品科学 ›› 2005, Vol. 26 ›› Issue (1): 116-120.

• 工艺技术 • 上一篇    下一篇

羊肚菌多糖提取、分离条件的选择

 武秋立, 安家彦   

  1. 天津大学化工学院; 大连轻工业学院生物与食品工程学院
  • 出版日期:2005-01-15 发布日期:2011-09-19

Study on Extraction and Separation of Polysaccharide of Morchella esculenta Cultured by Fermentation

 WU  Qiu-Li, AN  Jia-Yan   

  1. 1.College of Chemical Engineering and Technology,Tianjin University;2.College of Biology and Food Technology,Dalian Institute of Light Industry
  • Online:2005-01-15 Published:2011-09-19

摘要: 为了提高羊肚菌液体深层培养的多糖得率,本文研究了影响羊肚菌胞内、胞外多糖得率的各种因素。经试验确定羊肚菌胞外多糖分离优化条件为:沉淀温度-15℃,沉淀乙醇倍数3倍,沉淀时间24h,沉淀pH值为7。羊肚菌胞内多糖的浸提优化条件为:采用热水浸提,浸提温度85℃,浸提时间2h,浸提加水比50:1,浸提pH值为7,浸提次数两次。对比试验说明,优化效果显著。经SephadexG-150柱层析结果表明,羊肚菌胞外多糖由5种大分子多糖组成,而胞内多糖仅由2种组成。

关键词: 羊肚菌, 胞外多糖, 胞内多糖, 提取

Abstract: For improving the polysaccharide yield, the factors influencing the extraction and the separation of polysaccharide of Morchella esculenta cultured by submerged fermentation were studied. The optimum conditions of alcohol-precipitating exopolysaccharide were: 3 times alcohol; freezing time 24 hours; temperature -15℃ and pH 7. The optimum extracting conditions of endo-polysaccharides from mycelia were given below: adding 50 times volume water to dry mycelia; extracting time 120 min; temperature 85℃, pH 7 and extracting two times. The study proved that there were five kinds of components in exo- polysaccharides of Morchella esculenta and two kinds in endo-polysaccharides by Sephadex-150 column chromatograph.

Key words: Morchella esculenta, exopolysaccharide, endo-polysaccharide, extraction