食品科学 ›› 2005, Vol. 26 ›› Issue (3): 228-231.

• 营养卫生 • 上一篇    下一篇

菹草类胡萝卜素体外抗氧化活性的研究

 任丹丹, 彭光华, 王海滨, 张声华   

  1. 华中农业大学食品科技学院; 武汉工业学院食品科学与工程学院
  • 出版日期:2005-03-15 发布日期:2011-09-19

Study on Antioxidative Effects of Carotenoids Extracted from Potamogeton crispus L.in Vitro

 REN  Dan-Dan, PENG  Guang-Hua, WANG  Hai-Bin, ZHANG  Sheng-Hua   

  1. 1.College of Food Science and Technology, Huazhong Agricultural University; 2. College of Food Science and Engineering, Wuhan Polytechnic University
  • Online:2005-03-15 Published:2011-09-19

摘要: 菹草类胡萝卜素是红心鸭蛋中红色素的天然来源。本文用D—脱氧核糖法研究菹草类胡萝卜素清除·OH的效果,用硫代巴比妥酸法测定小鼠肝组织及肝线粒体中丙二醛含量,用分光光度法测定小鼠红细胞溶血和肝线粒体肿胀程度,从而研究类胡萝卜素的抗氧化效果。结果表明:菹草类胡萝卜素可以清除·OH,抑制·OH所致丙二醛的产生,减少红细胞溶血,减轻肝线粒体肿胀程度。可以得出菹草类胡萝卜素具有明显的抗氧化的作用。

关键词: 菹草, 类胡萝卜素, 羟基自由基, 抗氧化

Abstract: Carotenoids in Potamogeton crispus L. is a natural source of the red pigment in the red yolk of duck eggs. The scavenging effect of Carotenoids in Potamogeton crispus L. on hydroxyl radicals (·OH) was studied by D-deoxyrobise method. To study the anti-oxidative effect of Carotenoids in Potamogeton crispus L. the contents of MDA in tissue of liver and mitochondria of liver were studied by TBA assay in vitro, and the hemolysis extent of red blood cells and the swelling extent of mitochondria of liver were also detected by spectrophotometric methods. The result showed that Carotenoids in Potamogeton crispus L. had the ·OH scavenging activity. It could inhibit the generation of MDA, the swelling of mitochondria, and the hemolysis of red blood cells. The conclusion was that Carotenoids in Potamogeton crispus L. could have the effect of anti-oxidation.

Key words: Potamogeton crispus L., carotenoids, hydroxyl radical, anti-oxidation