食品科学 ›› 2005, Vol. 26 ›› Issue (3): 60-63.

• 基础研究 • 上一篇    下一篇

菱叶红景天多糖的提取、纯化鉴定及理化特性研究

 刘志伟, 姜华年   

  1. 中国疾病预防控制中心营养与食品安全所,武汉工业学院食品科学与工程学院,浙江丽水学院生物系
  • 出版日期:2005-03-15 发布日期:2011-09-19

Study on Extraction, Purification Identification and Physical and Chemical Characteristics Assay of Rhodiola Henryi Polysaccharides

 LIU  Zhi-Wei, JIANG  Hua-Nian   

  1. 1.Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention;2.College of Food Science and Engineering, Wuhan Polytechnic University; 3.Department of Biology, Lishui College
  • Online:2005-03-15 Published:2011-09-19

摘要: 以有效的热溶水提法,从菱叶红景天植物中得到粗多糖RHP。RHP经乙醇分级得三个级分RHP-I,RHP-II,RHPIII,取RHPIII脱蛋白,脱色,DEAD-纤维素柱层析,Saphadex G-200柱层析分离纯化,色谱鉴定及初步理化特性分析,得一纯度较高的均一多糖RHPS。PC分析其单糖组成有L-阿拉伯糖、L-鼠李糖、D-葡萄糖和另一个待确定的组分组成。

关键词: 菱叶红景天多糖, 提取, 纯化, 鉴定, 理化特性

Abstract: The R. Henryi Polysaccharides (RHP) were isolated from R. henryi by a high efficient water-extrating method. RHP- I,RHP-II and RHP-III were obtained by ethanol precipitating in RHP. After using RHP-III, eliminating albumen, decolorating, dialysis and freeze drying, it was purified by DEAE-cellulose column chromatography. As purified polysaccharides, RHPs were obtained and identified by Saphadex G-200 column chromatography and UV and IR spectrum.The results analysed by paper chromatography showed that its monnosaccharides were arahinose(Ara), rhamnose (Rha) and glucose(Glc), but a monnosaccharide should be further identified.

Key words: R.Henryi polysaccharides(RHP), extraction, purification, identification, physical and chemical charac- teristics