食品科学 ›› 2005, Vol. 26 ›› Issue (9): 215-218.

• 工艺技术 • 上一篇    下一篇

葛仙米藻胆蛋白提取工艺及藻蓝蛋白稳定性研究

 范刚, 陈德文, 潘思轶, 汪兴平   

  1. 华中农业大学食品科学技术学院,湖北民族学院生物科学与技术学院
  • 出版日期:2005-09-15 发布日期:2011-09-20

Study on Extraction Technology of Phycobiliproteins and Stability of Phycocyanins from Nostoc sphaeroids Kutz

 FAN  Gang, CHEN  De-Wen, PAN  Si-Yi, WANG  Xing-Ping   

  1. 1.College of Food Science and Technology, Huazhong Agricultural University; 2.College of Biological Science and Technology, Hubei Institute for Nationalities
  • Online:2005-09-15 Published:2011-09-20

摘要: 对葛仙米藻胆蛋白的提取工艺及其稳定性进行了研究。采用单因素实验优化葛仙米藻胆蛋白的提取工艺。结果表明,NaCl浓度为0.24mol/L,pH7及提取时间为4.2h时,藻胆蛋白的得率最高,分别为7.004%,7.438%,5.924%;藻蓝蛋白的稳定性实验表明,藻蓝蛋白在30℃以下,酒精浓度在10%以下,pH值在6~8的范围内比较稳定。

关键词: 葛仙米, 藻胆蛋白, 藻蓝蛋白, 提取, 稳定性

Abstract: Effect of one factor on extraction of phycobiliproteins from Nostoc sphaeroids Kutz and stability of phycocyanin were studied. The results show that concentration, pH value and extraction time of buffer affected the extraction rate of phycobiliproteins mostly. When concentration of buffer was 0.24mol/L, pH7, extraction time was 4.2h, the yield of phycobiliproteins was the highest, which was 7.004%, 7.438%, 5.924% respectively. And phycocyanin is stable under 30℃, 10% alcohol concentration and pH6~8.

Key words: Nostoc Sphaeroids Kutz, phycobiliproteins, phycocyanin, extraction, stability