食品科学 ›› 2007, Vol. 28 ›› Issue (1): 142-147.

• 工艺技术 • 上一篇    下一篇

富硒绿茶功能成分的超声波提取技术及其抗氧化活性研究

 王飞飞, 余芳, 辛志宏, 胡秋辉   

  1.  南京农业大学食品科技学院; 南京农业大学食品科技学院 江苏南京210095; 江苏南京210095;
  • 出版日期:2007-01-15 发布日期:2011-12-31

Study on Ultrasonic-assisted Extraction and Antioxidant Activity of Se-enriched Green Tea Functional Components

 WANG  Fei-Fei, YU  Fang, XIN  Zhi-Hong, HU  Qiu-Hui   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2007-01-15 Published:2011-12-31

摘要: 采用超声波技术提取富硒绿茶功能成分,通过L9(34)正交试验对提取工艺进行优化,采用DPPH法和亚油酸体系法对富硒绿茶功能成分抗氧化活性进行了评价,并分析了化学组成。结果表明按照工艺:50%乙醇为溶剂,超声波功率250W,料液比为1:15,提取温度50℃,提取时间1.5h,重复提取两次,制得的富硒绿茶功能成分得率为43.76%,其抗氧化活性高于其他处理制得的提取物和阳性对照α-生育酚,其化学组成为:硒4.35±0.11μg/g,茶多酚(55.84±2.31)%,多糖(13.37±3.02)%,蛋白(25.75±4.05)%,咖啡碱(5.99±0.01)%。

关键词: 富硒绿茶, 功能成分, 超声波, 抗氧化

Abstract: The ultrasonic was used to extract Se-enriched green tea functional components. The extraction pararmeters were optimized by L9(34) orthogonal design. And the composition of Se-enriched tea functional components was researched. The antioxidant activity of Se-enriched green tea functional components was assessed by FTC method and DPPH method. The optimized parameters were as follow: 50% (W/V) ethanol as solvent, ultrasonic power of 250W, ratio of solid/liquid of 1:15, extracting for 2 times at 50℃ for 1.5 hours each time. The yield was 43.76%. The antioxidant activity of the extracts under the optimized condition, which contained Selenium 4.35±0.11μg/g, tea polyphenol (55.84±2.31)%, polysaccharide (13.37± 3.02)%, protein (25.75±4.05)%, theine (5.99±0.01)%, was stronger than the extracts under other technology parameters and positivec ontrolα -tocopherol.

Key words: Se-enriched, ultrasonic, functional components, antioxidant