食品科学 ›› 2007, Vol. 28 ›› Issue (10): 520-522.

• 营养卫生 • 上一篇    下一篇

青钱柳多糖对人宫颈癌HeLa细胞和人脐带内皮细胞生长的影响

 刘昕, 王顺启, 谢明勇, 谢建华, 黄丹菲, 唐永富   

  1. 南昌大学食品科学与技术国家重点实验室; 南昌大学食品科学与技术国家重点实验室 江西南昌330047; 江西南昌330047;
  • 出版日期:2007-10-15 发布日期:2011-11-22

Effects of Polysaccharide from Cyclocarya paliurus (Batal.) Iljinskjk on Growth of HeLa Cells and Human Umbilical Vein Endothelial Cells

 LIU  Xin, WANG  Shun-Qi, XIE  Ming-Yong, XIE  Jian-Hua, HUANG  Dan-Fei, TANG  Yong-Fu   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2007-10-15 Published:2011-11-22

摘要: 目的:研究青钱柳多糖(polysaccharides isolated from Cyclocarya paliurus(Batal.)Iljinskjk,PCP)对人宫颈癌HeLa细胞和人脐带内皮细胞(HUVEC)生长的影响。方法:采用噻唑蓝(MTT)法,观察不同浓度、不同提取方法获得的PCP对HeLa细胞和HUVEC细胞生长的影响。结果:PCP I、II在50、100、200、400μg/ml浓度条件下,均可极显著性抑制HeLa细胞生长(p<0.01)。PCP I、II对HUVEC细胞生长有一定影响,但增殖抑制率不高;在PCP抑癌作用最高的浓度处(200μg/ml),PCP对HUVEC细胞的生长没有影响。结论:PCP极显著性抑制HeLa细胞的生长;在P C P抑癌作用最高的浓度处,P C P对H U V E C细胞的生长没有影响。

关键词: 青钱柳, 多糖, HeLa细胞, HUVEC细胞

Abstract: Objective: To investigate the regulate effects of Polysaccharides isolated from Cyclocarya paliurus (Batal.) Iljinskjk (PCP)on human cervical carcinoma HeLa cells and human umbilical vein endothelial cells(HUVEC) in vitro. Methods: MTT method was used to observe the effects of PCP, which were acquired by different extraction methods, on the HeLa and HUVEC cells respectively at different concentration. Results: PCP I and II can both inhibit the proliferation of HeLa cells extremely (p<0.01) at the concentration of 50, 100, 200, 400μg/ml. PCP I and II can influence the growth of HUVEC cells, but Inhibition of cell proliferation was not sharp; PCP can’t influence the growth of HUVEC cells at the concentration which the inhibitory rate of PCP is the most distinct on HELA cells (200μg/ml). Conclusions: PCP can significantly inhibit the proliferation of HeLa cells, and it can’t influence the growth of HUVEC cells at the concentration which the inhibitory rate of PCP is the highest on HeLa cells.

Key words: Cyclocarya paliurus (Batal.) Iljinskjk, polysaccharide, HeLa cells, HUVEC cells