食品科学 ›› 2007, Vol. 28 ›› Issue (11): 37-39.

• 基础研究 • 上一篇    下一篇

CdTe量子点标记铁蛋白的光谱研究

 庄红, 张梅, 孔祥贵, 刘静波   

  1. 吉林大学军需科技学院; 中科院长春光学精密机械与物理研究所激发态物理重点实验室; 吉林大学军需科技学院 吉林长春130062; 吉林长春130062; 吉林长春130033;
  • 出版日期:2007-11-15 发布日期:2011-11-22

Study on Spectra of CdTe Quantum Dots Labeling Ferritin

 ZHUANG  Hong, ZHANG  Mei, KONG  Xiang-Gui, LIU  Jing-Bo   

  1. 1.College of Quartermaster Technology, Jilin University, Changchun 130062, China; 2.Key Laboratory of Excited State Process, Changchun Institute of Optics Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China
  • Online:2007-11-15 Published:2011-11-22

摘要: 采用水相合成的CdTe量子点标记铁蛋白,利用荧光和飞秒技术系统地考察了标记前后体系的发射光谱和发光寿命的变化。与单独CdTe量子点相比,标记了铁蛋白的量子点发射光谱半峰宽不变,发光强度随着铁蛋白的量增加而降低,并且发光寿命没有改变。通过改变体系的pH值、铁蛋白量等各种条件验证了CdTe-Ferritin溶液发射光谱和发光寿命的变化确实是由于铁蛋白和CdTe量子点之间的结合反应引起的。

关键词: 铁蛋白, 量子点, 光谱

Abstract: The ferritin can be labeled with CdTe quantum dots synthesized in aqueous solution. The changes of emission spectra and lifetime of CdTe quantum dots before and after labeling ferritin were studied by UV and fluorescence spectrophotometries. The emission lifetime of CdTe quantum dots bound to ferritin remained unchanged, but the PL intensity was decreased with increasing amount of ferritin. The fact that the changes of emission intensity and luminescence lifetime are due to the binding of CdTe and ferritin is further confirmed through experiments by changing pH values and ferritin amount of test solution.

Key words: ferritin, quantum dot, spectra