食品科学 ›› 2007, Vol. 28 ›› Issue (2): 205-209.

• 生物工程 • 上一篇    下一篇

冬虫夏草液体发酵产多糖条件优化及饮料研制

 武忠伟, 王振河, 赵现方, 郭爱莲   

  1. 河南科技学院生物工程系; 西北大学生命科学学院 河南新乡453003; 河南新乡453003; 陕西西安710069;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Study on Optimization of Submerged Fermentation for Cordycepic Polysaccharides and Beverage Exploitation

 WU  Zhong-Wei, WANG  Zhen-He, ZHAO  Xian-Fang, GUO  Ai-Lian   

  1. 1.Department of Bioengineering, Henan Institute of Science and Technology, Xinxiang 453003, China; 2.College of Life Science, Northwest University, Xi’an 710069, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 研究了培养时间、初始pH值、乙醇浓度、二次补糖等因素对冬虫夏草菌丝生长和产糖的影响,得出了最佳培养时间为6d,初始pH为5.5,乙醇浓度为1.0%,二次补糖为复合碳源葡萄糖+甘露糖+半乳糖(3:4:2)1.2g/100ml,发酵液胞外多糖产量和菌丝干重最高分别可达5949.6mg/L和17.35g/L。功能性虫草饮料以添加枸杞+党参(2:1)15g/100ml发酵的配伍最佳。最优饮料配方:发酵液:水=70:30、蔗糖7%、柠檬酸0.1%、稳定剂0.15%。

关键词: 冬虫夏草, 深层培养, 多糖, 饮料

Abstract: The effects of culture time, initial pH, ethanol concentration and secondary supplementing sugar on the biomass and the polysaccharides production of Cordyceps sinensis were studied. The results showed:the optimum culture time 6d, initial pH 5.5, ethanol thickness 1.0% and secondary supplementing sugar Glu+Man+Gal(3:4:2)1.2g/100ml. The maximal output of polysaccharides and the mycelium weight reached 5949.6mg/L and 17.35g/L respectively. The optimum functions obtained whencordycepic beverage was added with Lycium barbarum and Codonopsis pilosula (2:1) 15g/100ml. The best beverage formula is fermentative fluid:water=70:30, sucrose 7%, citric acid 0.1% and stability 0.15%.

Key words: Cordyceps sinensis, submerged culture, polysaccharides, beverage