HPLC法测定油菜蜂花粉中黄酮含量及六种破壁方法对黄酮提取的影响

食品科学 ›› 2008, Vol. 29 ›› Issue (10): 455-458.

HPLC法测定油菜蜂花粉中黄酮含量及六种破壁方法对黄酮提取的影响

阮征, 邓泽元, 吴龙耀, 谢明勇, 吴谋成

南昌大学食品科学与技术国家重点实验室；南昌大学生命科学学院；华中农业大学食品科学技术学院；

出版日期:2008-10-15 发布日期:2011-12-08

Determination of Flavonoids Content in Rape Bee Pollen by HPLC and Effects of Six Cell Wall-breaking Techniques on Flavonoids Release from Rape Bee Pollen Cell

RUAN Zheng, DENG Ze-Yuan, WU Long-Yao, XIE Ming-Yong, WU Mou-Cheng

1.State Key Laboratory of Food Science and Technology,Nanchang University,Nanchang 330047,China; 2. College of Life Science,Nanchang University,Nanchang 330031,China; 3.College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

Online:2008-10-15 Published:2011-12-08

摘要/Abstract

摘要： 目的:六种破壁方法处理油菜蜂花粉,运用HPLC测定花粉中的黄酮含量,研究不同破壁方法对黄酮提取的影响。方法:色谱柱为反相C18柱,流动相V(甲醇):V(0.4%磷酸)为60:40,流速0.5ml/min,波长370nm。采用超声法、酶解法、温差法、温差-超声法、温差-酶法和混合方法进行花粉破壁。结果:未破壁花粉总黄酮含量为2.2447mg/g。六种破壁方法提取的花粉总黄酮含量比未破壁的依次增加12.4%、8.3%、23.1%、25.4%、28.1%、31.5%,破壁分别为52.56%、41.31%、76.94%、78.66%、83.13%、86.88%。结论:建立了HPLC测定花粉黄酮的方法;花粉破壁率越高,黄酮的提取得率越高。

关键词: 油菜花粉, 黄酮, 破壁, 高效液相色谱

Abstract: Objective: To determine flavonoids content in rape bee pollen by HPLC and analyze the effects of six cell wall-breaking techniques on flavonoids extraction from rape bee pollen. Methods: The HPLC conditions were as follows: reversed-phase C18 column as separation column,methanol:0.4% phosphoric acid (60:40) as mobile phase,detection wavelength 370 nm,quercetin and kaempferol as external standards and flow rate 0.5 ml/min.The adopted wall-breaking techniques were ultrasonic method,enzymolysis method,temperature difference method,temperature difference-ultrasonic method,temperature difference-enzymolysis method and integrated method of the threee single methods. Results: The flavonoids content of original rape bee pollen is 2.2447 mg/g by HPLC. The flavonoids contents of wall-broken rape bee pollen by the above-mentioned methods are higher 12.4%,8.3%,23.1%,25.4%,28.1% and 31.5% than 2.2447 mg/g respectively,and the wall-breaking rates of these methods are 52.66%,41.31%,76.94%,78.66%,83.13% and 86.8% respectively. Conclusion: The HPLC method can provide scientific indexes for developing functional components of rape bee pollen. The higher wall-breaking rate,the more flavonoids released from rape bee pollen.

Key words: rape bee pollen, flavonoids, wall-breaking, HPLC

阮征, 邓泽元, 吴龙耀, 谢明勇, 吴谋成. HPLC法测定油菜蜂花粉中黄酮含量及六种破壁方法对黄酮提取的影响[J]. 食品科学, 2008, 29(10): 455-458.

RUAN Zheng, DENG Ze-Yuan, WU Long-Yao, XIE Ming-Yong, WU Mou-Cheng. Determination of Flavonoids Content in Rape Bee Pollen by HPLC and Effects of Six Cell Wall-breaking Techniques on Flavonoids Release from Rape Bee Pollen Cell[J]. FOOD SCIENCE, 2008, 29(10): 455-458.

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