食品科学 ›› 2008, Vol. 29 ›› Issue (11): 505-509.

• 分析检测 • 上一篇    下一篇

乳酸菌对酸汤挥发性物质的影响

 徐俐, 戴岳宗   

  1. 贵州大学食品科学系;
  • 出版日期:2008-11-15 发布日期:2011-12-08

Effects of Different Lactic Acid Bacteria in Sour Soup on Volatile Flavor Compounds

 XU  Li, DAI  Yue-Zong   

  1. Department of Food Science,Guizhou University,Guiyang 550025,China
  • Online:2008-11-15 Published:2011-12-08

摘要: 用嗜酸乳杆菌(L.acidophilus)、植物乳杆菌(L.plantarun)以及两种菌种的混合菌发酵酸汤和传统的自然发酵酸汤进行比较,采用蒸馏萃取法提取酸汤中产生的挥发性物质,分别用GC-MS分析了挥发性成分。结果表明:四个处理样品中的挥发性化合物有92种,其中自然发酵、La、Lp、混合处理分别为55、65、58和52种,这些化合物包括醇(20)、酸(6)、酯(17)、醛(5)、酮(2)、烯(29)、含硫化合物(1)、烷烃(4)、其他化合物(6)等9类。以混合处理组总挥发物含量最高,为96.273%,其次为嗜酸乳杆菌组,再次为自然发酵组,最少为植物乳杆菌组,但四种处理都以酸含量为最高,说明酸汤产品主体风味是酸类物质。

关键词: 酸汤, 乳酸菌, 挥发物质, 气质联用

Abstract: L.acidophilus,L.plantarun and their mixture were inoculated into the sour soup made from chopped tomato,pepper,ginger and garlic for fermentation at 25 ℃ respectively.The volatile compounds in the three sour soups and natural fermented sour soup were extracted by means of distillation extraction method,and they were analyzed with gas chromatography and mass spectrometry technique(GC-MS).Results showed that the volatile compounds are found to be 92 kinds in the above four sour soups in number,which are 65,58,55 and 52 kinds in them respectively.These compounds consist of 9 kinds,namely alcohols(20),acids(6),esters(17),aldehydes(5),ketones(2),alkenes(29),sulfurcompounds(1),alkanes(4) and other compounds(6).The total volatiles content in the fermented sour soup by the mixture of L.acidophilus and L.plantarun is the highest,reaching 96.273%,followed by the fermented sour soup by L.acidophilus,the natural fermented sour soup and the fermented sour soup by L.plantarun in turn.However the acid contents in the four sour soups are high,which indicated that the main flavor compounds in the sour soup products are acids.

Key words: sour soup, lactic acid bacteria, volatile compounds, gas chromatography-mass spectrometry(GC-MS)