食品科学 ›› 2008, Vol. 29 ›› Issue (9): 260-263.

• 工艺技术 • 上一篇    下一篇

酶法提取牛蒡多糖的研究

 高明侠, 苗敬芝, 曹泽虹, 董玉玮, 吕兆启   

  1. 徐州工程学院生物工程学院;
  • 出版日期:2008-09-15 发布日期:2011-12-08

Study on Extraction of Burdock Polysaccharides by Double-enzyme Method

 GAO  Ming-Xia, MIAO  Jing-Zhi, CAO  Ze-Hong, DONG  Yu-Wei, 吕Zhao-Qi   

  1. College of Bioengineering,Xuzhou Institute of Technology,Xuzhou 221008,China
  • Online:2008-09-15 Published:2011-12-08

摘要: 本实验以牛蒡为原料,采用双酶法提取牛蒡多糖,并对其工艺条件进行探讨。结果表明:最佳提取条件为木瓜蛋白酶和植物水解蛋白酶各2%,酶解温度45℃,酶解时间4h,pH8.0,固液比1:15。采用双酶水解后,牛蒡多糖提取率为12.40%。

关键词: 牛蒡, 双酶法, 提取, 多糖

Abstract: The optimized extraction conditions of polysaccharides from burdock with double-enzymes by orthogonal test are as follows: respective quantity of papain and plant hydrolytic proteinase 2%, temperature 45℃, respective reaction time of the double enzymes 4 h, pH 8.0 and ratio of solid-liguid 1:15. Under the conditions, the extraction rate of burdock pohysaccharides is 12.40%.

Key words: burdock, double-enzymatic method, extraction, polysaccharides ..