食品科学 ›› 2008, Vol. 29 ›› Issue (9): 437-440.

• 生物工程 • 上一篇    下一篇

金针菇深层发酵条件及水浴提取菌丝体多糖的研究

 刘晓鹏, 姜宁, 向东山, 周大寨, 郑小江   

  1. 生物资源保护与利用湖北省重点实验室; 湖北民族学院生物科学与技术学院;
  • 出版日期:2008-09-15 发布日期:2011-12-08

Study on Submerged Fermentation Conditions of Flammuling velutipes and Extraction of Mycelial Polysaccharides by Water Bath

 LIU  Xiao-Peng, JIANG  Ning, XIANG  Dong-Shan, ZHOU  Da-Zhai, ZHENG  Xiao-Jiang   

  1. 1. Hubei Key Laboratory of Biological Resource Conservation and Utilization,Enshi 445000,China; 2. School of Biological Science and Technology,Hubei Institute for Nationalities,Enshi 445000,China
  • Online:2008-09-15 Published:2011-12-08

摘要: 目的:研究金针菇深层发酵的条件、培养菌丝体及菌丝体多糖的提取工艺。方法:通过发酵罐发酵研究金针菇深层发酵的条件及培养菌丝体;通过单因素试验及正交试验研究菌丝体多糖的水浴提取工艺。结果:试验确定了金针菇发酵的最佳种龄为48h、最适接种量是15%,菌丝生长曲线测定确定了发酵周期为培养时间96h,溶氧30%~50%,25℃培养;水浴提取金针菇菌丝体多糖的最佳工艺是:料液比1:60,水浴时间90min,水浴温度60℃。结论:明确了金针菇深层发酵的培养条件,通过发酵罐发酵每升发酵液可得到40g金针菇菌丝体(干重);得到了金针菇菌丝体多糖的水浴提取工艺。

关键词: 金针菇, 深层发酵, 多糖, 提取

Abstract: The experiment results showed that the optimum conditions of submerged fermentation are as follows: optimal seed age 48 h, inoculation size 15%, culture temperature 25 ℃ and fermentation time 96 h. The optimum conditions for extracting polysaccharides from Flammuling velutipes mycelia by water bath extraction method are as follows: material-liquid ratio 1:60, temperature 60 ℃ and time of 90 minutes.

Key words: Flammuling velutipes, submerged fermentation, polysaccharides, extraction