食品科学 ›› 2010, Vol. 31 ›› Issue (17): 18-21.doi: 10.7506/spkx1002-6630-201017004

• 基础研究 • 上一篇    下一篇

富硒金针菇对自由基清除作用

李华为1,赵素云2,铁 梅2,*   

  1. 1.沈阳师范大学化学与生命科学学院
    2.辽宁大学环境学院
  • 收稿日期:2010-01-11 出版日期:2010-09-15 发布日期:2010-12-29
  • 通讯作者: 铁 梅 E-mail:tiemei07@yahoo.cn
  • 基金资助:

    国家自然科学基金项目(20611130317/B01);辽宁省教育厅科学技术研究项目(2008227)

Scavenging Effect of Selenium-rich Flammulina velutipes on Hydroxyl Free Radicals

LI Hua-wei1,ZHAO Su-yun2,TIE Mei2,*   

  1. 1. College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034, China;
    2. School of Environmental Science, Liaoning University, Shenyang 110036, China
  • Received:2010-01-11 Online:2010-09-15 Published:2010-12-29
  • Contact: TIE Mei E-mail:tiemei07@yahoo.cn

摘要:

采用现代分离技术从富硒金针菇中提取硒多糖和硒蛋白,采用苯酚- 硫酸法、ICP-MS 法和考马斯亮蓝染色法对硒多糖中多糖含量、硒含量及硒蛋白中蛋白和硒的含量进行测定;在Fenton 体系的最佳实验条件下,研究硒多糖和硒蛋白对羟自由基的清除作用。结果表明:硒多糖和硒蛋白清除羟自由基的效果比与之相同浓度的无机硒和相同浓度的多糖及蛋白都要明显;其中,硒多糖的最高清除率可达65.05%,而硒蛋白的最高清除率可达19.67%。

关键词: 金针菇, 硒多糖, 硒蛋白, 羟自由基

Abstract:

The fruit bodies of selenium-rich Flammulina velutipes were extracted by modern separation techniques to obtain Se-binding polysaccharides and Se-binding proteins. Phenol-sulfuric acid method, ICP-MS and Coomassie brilliant blue G-250 dye method were used for the quantifications of polysaccharides, selenium and proteins, respectively. The scavenging effects of the Se-binding polysaccharide extract and the Se-binding protein extract on hydroxyl free radicals generated in a Fenton system were evaluated under optimal experimental conditions. It was found that the hydroxyl free radical scavenging effects of Sebinding polysaccharides and Se-binding proteins were more obvious than those of inorganic selenium, pure polysaccharides or pure proteins at the same concentrations. The maximum scavenging rates of Se-binding polysaccharides and Se-binding proteins on hydroxyl free radicals were 65.05 % and 19.67%, respectively.

Key words: Flammulina velutipes, Se-binding polysaccharides, Se-binding proteins, hydroxyl free radicals

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