食品科学 ›› 2010, Vol. 31 ›› Issue (5): 237-239.doi: 10.7506/spkx1002-6300-201005053

• 生物工程 • 上一篇    下一篇

一磷酸腺苷活化蛋白激酶对羊肉糖酵解的影响

马 霞,李 泽,靳 烨*   

  1. 内蒙古农业大学食品科学与工程学院
  • 收稿日期:2009-07-24 修回日期:2009-10-31 出版日期:2010-03-01 发布日期:2010-12-29
  • 通讯作者: 靳 烨 E-mail:jinyeyc@yahoo.com.cn

Effect of AMP-activated Protein Kinase on Glycolysis in Mutton

MA Xia,LI Ze,JIN Ye*   

  1. (College of Food Science and Engineering, Inner Mongolia Agricultural University, Huhhot 010018, China)
  • Received:2009-07-24 Revised:2009-10-31 Online:2010-03-01 Published:2010-12-29
  • Contact: JIN Ye E-mail:jinyeyc@yahoo.com.cn

摘要:

通过抑制剂阿糖腺苷(araA)改变肉样中的一磷酸腺苷活化蛋白激酶(AMPK)活性后,测定羊肉样品中乳酸含量、己糖激酶(HK)活性及pH 值的变化,研究AMPK 活性变化对羊肉宰后糖酵解的影响。结果表明:AMPK活性降低时,乳酸蓄积率降低(P < 0.01),己糖激酶活性受抑制(P < 0.01),pH 值降速减慢(P < 0.01),并且3 个指标随AMPK 活性变化而表现出规律性变化。

关键词: AMPK, 羊肉, 糖酵解

Abstract:

In order to investigate the effect of AMP-activated protein kinase (AMPK) on glycolysis in biceps femoris muscle from postmortem Mongolia sheep carcases, lactic acid concentration, hexokinase activity and pH change were determined after the inhibition of AMPK activity in mutton muscle samples by araA. Results indicated that the inhibition of AMPK activity resulted in a significant decrease in lactic acid accumulation (P<0.01), an obvious inhibition of hexokinase activity (P<0.01), and a great reduction in the rate of pH decrease (P<0.01). Moreover, a regular change in three indices was also observed due to the change of AMPK activity.

Key words: AMPK, mutton, glycolysis

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