食品科学 ›› 2011, Vol. 32 ›› Issue (9): 51-54.doi: 10.7506/spkx1002-6630-201109011

• 基础研究 • 上一篇    下一篇

不同方法制备的葵花籽多肽酶解物对亚硝化反应的抑制作用

王海凤,王常青,刘佳璐,吕鹏,赵陈勇,许洁   

  1. 1. 山西大学生命科学学院 2. 山西大学化学化工学院
  • 出版日期:2011-05-15 发布日期:2011-04-11

Anti-nitrosation Effects of Sunflower Seed Peptides Prepared by Different Enzymatic Hydrolysis Methods

WANG Hai-feng1,WANG Chang-qing1,*,LIU Jia-lu2,LU Peng1,ZHAO Chen-yong1,XU Jie1   

  1. 1. College of Life Science, Shanxi University, Taiyuan 030006, China; 2. College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
  • Online:2011-05-15 Published:2011-04-11

摘要: 以葵花籽分离蛋白为原料,研究不同方法制备的葵花籽多肽(SSP)对亚硝化反应的抑制作用。结果表明,与无超声波协同的常规酶解方法相比,在超声波振荡功率120W、超声时间/间歇时间为3min/7min的条件下,用酸性蛋白酶水解葵花籽蛋白,分别可以提高水解度和多肽得率17.3%和16.2%。用此方法得到的酶解产物对亚硝化反应具有更强的抑制作用,对亚硝酸钠的最大清除率和对亚硝胺的最大阻断率分别达到84.5%和92.4%,其效果均优于VC和谷胱甘肽。

关键词: 葵花籽多肽, 亚硝酸盐, 亚硝胺, 抑制作用

Abstract: Sunflower seed peptides (SSPs) prepared from sunflower protein isolate by sequential two-step enzymatic hydrolysis with alkaline protease and acidic protease with and without ultrasonic assistance (used in the second stage) were assessed for their anti-nitrosation effects. Compared to the conventional hydrolysis method without ultrasonic assistance, the degree of hydrolysis and the yield of peptides resulting from ultrasonic treatment at 120 W power and 3:7 working time-to-intermission time ratio were increased by 17.3% and 16.2%, respectively. SSPs obtained by the ultrasonic-assisted method had more powerful inhibitory effect on nitrosation than those obtained by the conventional method, vitamin C and glutathione, and the maximum scavenging rate of sodium nitrite and blocking rate of nitrosamine synthesis were up to 84.5% and 92.4%, respectively.

Key words: sunflower seed peptides, nitrite sodium, nitrosamine, inhibitory effect

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