土党参多糖不同提取方法的比较研究

食品科学 ›› 2012, Vol. 33 ›› Issue (18): 45-48.

土党参多糖不同提取方法的比较研究

吴兰芳，景永帅，张振东，杨娟*

贵州省、中国科学院天然产物化学重点实验室

收稿日期:2011-07-07 修回日期:2012-07-23 出版日期:2012-09-25 发布日期:2012-09-07
通讯作者: 杨娟 E-mail:yangxz2002@126.com
基金资助:
贵州省中药现代化科技产业研究开发专项项目基金

A Comparative Study of Different Extraction Methods for Polysaccharides from Campanumoea javanica Roots

Received:2011-07-07 Revised:2012-07-23 Online:2012-09-25 Published:2012-09-07
Contact: Yang Juan E-mail:yangxz2002@126.com

摘要/Abstract

摘要： 目的：比较3种提取方法对土党参原料多糖提取率的影响。方法：以土党参多糖提取率为考察指标，对热水浸提、超声波以及微波辅助3种提取方法进行均匀优化试验设计，对多糖提取率进行二次多项式逐步回归分析。结果：热水浸提法的优化条件为液料比25:1(mL/g)、温度70℃、提取时间60min，提取率28.05%；超声波提取法的优化条件为液料比50:1、功率210W、提取时间40min，提取率28.12%；微波法提取的优化条件为液料比15:1、功率900W、提取时间5min，提取率28.99%。结论：3种提取方法对多糖提取率影响差异不明显，但超声波和微波辅助提取较热水浸提具有省时的优点。

关键词: 土党参, 多糖, 热水浸提, 超声波, 微波, 均匀设计

Abstract: Objective: To compare the extraction efficiencies of polysaccharides from Campanumoea javanica roots by different extraction methods. Methods: Three different extraction methods were optimized by uniform design combined with second-order polynomial regression analysis for enhanced extraction efficiency. Results: The optimal hot water extraction conditions were material-to-liquid ratio of 1:25 (g/mL), extraction temperature of 70 ℃ and extraction time of 60 min. Under these conditions, the maximum yield of polysaccharides was 28.05%. The optimal ultrasonic-assisted extraction conditions were material-to-liquid ratio of 1:50 (g/mL), ultrasonic power of 210 W and extraction time of 40 min. Under these conditions, the maximum yield of polysaccharides was 28.12%. The optimal microwave-assisted extraction conditions were material-to-liquid ratio of 1:15 (g/mL), microwave power of 900 W and extraction time of 5 min. Under these conditions, the maximum yield of polysaccharides was 28.99%. Conclusion: No significant difference was observed among three extraction methods. However, ultrasonic-assisted extraction and microwave-assisted extraction have a time-saving advantage over hot water extraction.

Key words: Campanumoea javanica, polysaccharide, hot water extraction, ultrasound-assisted extraction, microwave-assisted extraction, uniform design

中图分类号:

S567.9

吴兰芳，景永帅，张振东，杨娟*. 土党参多糖不同提取方法的比较研究[J]. 食品科学, 2012, 33(18): 45-48.

参考文献

[1]何顺志, 徐文芬..贵州中草药资源研究[J].贵州科技出版社,2007,:653-653
[2]杨小生, 张占军, 杨再昌, 等. .土党参多糖衍生物及其制备方法和应用[J].专利,2005,(1648136):-
[3]张振东, 杨娟, 吴兰芳, 等.土党参多糖对小鼠脑缺血再灌注损伤的保护作用[J].中国药理学通报,2011,27(4):508-510
[4]张振东, 杨娟, 吴兰芳, 等..NGF样土党参多糖促进小鼠学习记忆作用的实验研究[J].时珍国医国药,2011,(第6期或第9期):-
[5]ZHI Y G, HAN X X, & LI J R.Ultrasound-assisted extraction of polysaccharides from mulberry leaves[J].Food Chemistry,2011,(3):1273-1279
[6] CHEMAT F, ZILL-E-HUMA, KHAN M K. Applications of ultrasound in food technology: Processing, preservation and extraction. [J].Ultrasonics Sonochemistry,2011,18:813-835
[7]王莉, 陈正行, 张兵.不同提取方法提取米糠多糖工艺的优化研究[J].食品科学,2007,28(4):112-116
[8]WANG, J L, ZHANG J, ZHAO, B T A comparison study on microwave-assisted extraction of Potentilla anserinaL.polysaccharides with conventional method: Molecule weight and antioxidant activities evaluation[J].Carbohydrate Polymers,2010,80:84-93
[9]程力惠, 王建壮, 卢丽霞.正交设计优选巴戟天中多糖提取工艺[J].中药材,2010,33(1):125-127
[10]KSHIRSAGAR, A C, SINGHAL, R S.Optimization of starch oleate derivatives from native corn and hydrolyzed corn starch by response surface methodology[J].Carbohydrate Polymers,2007,69:455-461
[11]王钦德, 杨坚. .食品试验设计与统计分析. [J].中国农业大学出版社,2003,:368-369
[12]郭东星, 仇丽霞, 张满栋.均匀设计方法及其应用[J].数理医药学杂志,2005,18(1):112-116
[13]WANG Z J, LUO D H, NA C A.Optimization of polysaccharides extraction from Gynostemma pentaphyllum Makino using Uniform Design[J].Carbohydrate Polymers,2007,69:311-317
[14]罗巅辉.均匀设计法优化超声波提取牛蒡多糖工艺的研究[J].河南师范大学学报,2008,36(1):115-117
[15]YANG C X, HE N, LING X P. .The isolation and characterization of polysaccharides from longan pulp [J].,2008,63:226-230
[16] 张惟杰. 糖复合物生化研究技术(第二版). [J].浙江大学出版社,1999,:11-11