食品科学 ›› 2012, Vol. 33 ›› Issue (22): 95-98.

• 工艺技术 • 上一篇    下一篇

大麦芽根蛋白提取工艺条件优化

阚俊鹏1,阮长青1,*,王立东2,王鹤霖1,孙书祥3   

  1. 1.黑龙江八一农垦大学食品学院 2.黑龙江省农产品加工工程技术研究中心(大庆) 3.哈尔滨龙垦麦芽有限公司
  • 收稿日期:2011-09-06 修回日期:2012-10-22 出版日期:2012-11-25 发布日期:2012-11-20
  • 通讯作者: 阮长青 E-mail:cqruan@163.com
  • 基金资助:

    黑龙江省教育厅科学技术研究项目计划

Optimization of Extraction Conditions for Protein from Barley Malt Roots

,Chang-Qing Ruan   

  • Received:2011-09-06 Revised:2012-10-22 Online:2012-11-25 Published:2012-11-20
  • Contact: Chang-Qing Ruan E-mail:cqruan@163.com

摘要: 为充分利用大麦芽生产的副产物——大麦芽根中的蛋白资源,采用国家标准方法测定麦芽根的主要成分,通过正交试验研究碱法提取麦芽根蛋白的最佳工艺条件。结果表明:麦芽根的蛋白质、灰分、水分、脂肪及粗纤维质量分数分别为29.2%、6.9%、6.42%、1.4%和10.7%。正交试验结果表明,麦芽根中蛋白提取的最佳工艺条件为浸提液pH9.0、料液比1:25(g/mL)、浸提时间60min、浸提温度40℃,在此条件下麦芽根蛋白质的浸提率为63.5%。该工艺提取麦芽根蛋白简便且提取率高。

关键词: 大麦芽根, 蛋白质, 提取, 正交试验

Abstract: Barley malt roots as a byproduct of malt product was analyzed for proximate chemical composition according to the national standards. Meanwhile, the extraction of alkali-soluble protein from barley malt roots was optimized by orthogonal array design method. Barley malt roots contained 29.2% protein, 6.9% ash, 6.42% water, 1.4% fat and 10.7% crude fiber. The optimal conditions for protein extraction were found to be extraction at 40 ℃ and pH 9.0 for 60 min with a solid-to-solvent ratio of 1:25 (g/mL), resulting in a protein yield of 63.5%. The optimized procedure provided a simple and high-yield method for protein extraction from barley malt roots.

Key words: barley malt roots, protein, extraction, orthogonal array design

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