食品中蛋白质检测技术研究进展

摘要/Abstract

摘要： 精确地检测食品中的蛋白质含量对质量管理、科学研究等都是必不可少的。本文在综述食品中蛋白质含量检测方法的原理、特点及应用范围的基础上，介绍最新的研究发展动态，为研究者在蛋白质检测方法选择或进一步改进和发展方面提供依据，以使各种方法得到更好的应用，从而提高食品中蛋白质检测的准确性。

关键词: 食品, 蛋白质检测, 方法, 发展

Abstract: Accurate determination of protein in foods is necessary for quality management and scientific research. The principle, characteristics and application of analytic methods for protein contents are reviewed in this article. Meanwhile, the future development trends are also discussed. These investigations will provide a theoretical reference for improving and developing suitable methods of protein detection in foods.

Key words: food, protein detection, methods, development

中图分类号:

TS207.3

孙?蓉，吴文标*. 食品中蛋白质检测技术研究进展[J]. 食品科学, 2012, 33(23): 393-398.

参考文献

[1] WEI Q, WU D, DU B,et al. A SpectrophotometricMethod for Determination of Total Proteins in Cow Milk Powder Samples Using the o-Nitrophenylfluorone/Mo (VI) Complex [J]. Journal of Food Composition and Analysis, 2006, 19 (1): 76-82.
[2] DOMINI C, VIDAL L, CRAVOTTO G,et al. A Simultaneous, Direct Microwave/Ultrasound-assisted Digestion Procedure for the Determination of Total Kjeldahl Nitrogen [J]. Ultrasonics Sonochemistry, 2009, 16(4): 564-569.
[3] JUNSOMBOON J, JAKMUNEE J. Flow Injection Conductometric System with Gas Diffusionseparation for the Determination of Kjeldahl Nitrogen in Milk and Chicken Meat [J]. Analytica Chimica Acta, 2008, 627(2): 232-238.
[4] BELJKA? B, MATI? J, MILOVANOVI? I, et al. Rapid Method for Determination of Protein Content in Cereals and Oilseeds: Validation, Measurement Uncertaintyand Comparison with the Kjeldahl Method [J]. Accreditation and Quality Assurance, 2010, 15(10): 555-561.
[5] WU W B. Recovery of Proteins from Potato Juice [D].Reading: University of Reading, 1991.
[6] ETHERIDGE R D, PESTI G M, Foster E H. A Comparison of Nitrogen Values Obtained Utilizing the Kjeldahl Nitrogen and Dumas Combustion Methodologies (Leco CNS 2000) on Samples Typical of an Animal Nutrition Analytical Laboratory [J]. Animal Feed Science and Technology, 1998, 73 (1-2): 21-28.
[7] SAINT-DENIS T, GOUPY J. Optimization of a Nitrogen Analyser Based on the Dumas Method [J]. Analytica Chimica Acta, 2004,515 (1): 191-198.
[8] HAKODA A, YUSUKE II, NAITO S,et al. Determination of Crude Protein in Macaroni Products by the Combustion Method and Comparison with the Kjeldahl Method: Interlaboratory Study [J]. Food Science and Technology Research, 2011, 17(3): 227-232.
[9] MOORE J C, DEVRIES J W, Lipp M, et al. Total Protein Methods and Their Potential Utility to Reduce the Risk of Food Protein Adulteration [J]. Comprehensive Reviews in Food Science and Food Safety, 2010, 9(4): 330-357.
[10] ROUCH D A, ROGINSKI H, BRITZ M L, et al. Determination of a Nitrogen Conversion Factor for Protein Content in Cheddar Cheese [J]. International Dairy Journal, 2008, 18 (2): 216-220.
[11] SALO-VIIIINIINEN P P, KOIVISTOINEN P E. Determination of Protein in Foods: Comparison of Net Protein and Crude Protein (N×6.25) Values [J]. Food Chemistry, 1996, 51(1): 21-31.
[12] KAMIZAKEA N K K, GONCALVESA M M, ZAIA C T B V, et al. Determination of Total Proteins in Cow Milk Powder Samples: a Comparative Study Between the Kjeldahl Method and Spectrophotometric Methods [J]. Journal of Food Composition and Analysis, 2003, 16 (4): 507-516.
[13] MARIA R C I, MARIA F V M, KáSSIO M G. L. Classification and Determination of Total Protein in Milk Powder Using Near Infrared Reflectance Spectrometry and the Successive Projections Algorithm for Variable Selection [J]. Vibrational Spectroscopy, 2011.
[14] EZEAGU I E, PETAKE J K, METGES C C,et al. Seed Protein Contents and Nitrogen-to-protein Conversion Factors for Some Uncultivated Tropical Plant Seeds [J]. Food Chemistry, 2002, 78 (1): 105-109.
[15] 鲁健章，孙丽华，周彦钢.凯氏定氮法测定鱼蛋白质含量的干扰因素分析[J].食品科学，2011，31(19)：453-456.
[16] CAPITáN-VALLVEYA L F, DUQUEB O, MIRóN-GARCIAA G, et al. Determination of Protein Content Using a Solid Phase Spectrophotometric Procedure [J]. Analytica Chimica Acta, 2001, 433 (1):155-163.
[17] OKUTUCU B, DINCER A, HABIB O, et al. Comparison of Five Methods for Determination of Total Plasma Protein Concentration [J]. Journal of Biochemistry Biophysical Methods, 2007, 70 (5): 709-711.
[18] LINDEBOOM N, WANASUNDARA P K J P D. Interference of Phenolic Compounds in Brassica Napus, Brassica Rapa and Sinapis Alba Seed Extracts with the Lowry Protein Assay [J]. Food Chemistry, 2007, 104 (1): 30-38.
[19] SMITH M R, PENNERT M H, SAMUEL E B. et al. Quantitative Colorimetric Assay for Total Protein Applied to the Red Wine Pinot Noir [J]. Journal of Agricultural and Food Chemistry, 2011, 59(13): 6871-6876.
[20] ANJALIE F, JEFFREY F. Melamine and Cyanuric Acid Do not Interfere with Bradford and Ninhydrin Assays for Protein Determination [J]. Food Chemistry, 2010, 121 (3): 912-917.
[21] BANIK S P, PAL S, Ghorai S, et al. Interference of Sugars in the Coomassie Blue G Dye Binding Assay of Proteins [J]. Analytical Biochemistry, 2009,386 (1): 113-115.
[22] GIANFRANCO G, CLAUDIO B, CRISTINA G. Inaccuracy of the Bradford Method for the Determination of Protein Concentration in Steroid-Horseradish Peroxidase Conjugates [J]. Analytica Chimica Acta, 1997, 337 (1): 93-97.
[23] ROMO C R,LAKIN A L,ROLFE E J. Properties of Protein Isolates Prepared from Ground Seeds Ⅰ.Development and Evaluation of a Dye Binding Procedure for the Measurement of Protein Solubility[J]. International Journal of Food Science & Technology, 1975, 10(5): 541-546.
[24] WU W B, LAKIN A L. Estimation of Protein in Potato Tissue by Dye Binding [J]. Food Chemistry, 1993, 46(1):49-53.
[25] KHAN M. I. Lysine Estimation with the Modified Udy-dye Binding Method in Hexaploid Wheat [J]. Cellular and Molecular Life Sciences, 1977, 34(6): 711-712.
[26] AMAMCHARLA J K, METAGER L E. Evaluation of a Rapid Protein Analyzer for Determination of Protein in Milk and Cream. Journal of Dairy Science, 2010, 93(8):3846-3857.
[27] LAKIN A L. Determination of Nitrogen and Estimation of Protein in Food [M]. London: science publishers, 1978.
[28] MANSO M A, MIGUEL M, LóPEZ-FANDI?O R. Application of Capillary Zone Electrophoresis to the Characterisation of the Human Milk Protein Profile and its Evolution throughout Lactation[J]. Journal of Chromatography A, 2007, 1146 (1): 110-117.
[29] BEAN S R, BIETZ J A, Lookhart G L. High-performance Capillary Electrophoresis of Cereal Proteins [J]. Journal of Chromatography A, 1998, 814 (1-2): 25-41.
[30] DONG Y Y. Capillary electrophoresis in food analysis [J]. Trends in Food Science & Technology, 1999, 10 (3): 87-93.
[31] MIRALLES B, RAMOS M, AMIGO L. Influence of Proteolysis of Milk on the Whey Protein to Total Protein Ratio as Determined by Capillary Electrophoresis [J]. Journal of Dairy Science, 2003, 86(9): 2813-2817.
[32] 栾吉梅，张晓东.有机染料共振光散射法测定蛋白质的研究进展[J].理化检验-化学分册，2007，43(2)：159-163.
[33] 梁金虎，唐英，张进，等.共振光散射技术的原理与研究进展[J].重庆文理学院学报(自然科学版)，2010，29(4)：31-36.
[34] FENG S L, PAN Z H, FAN J. Determination of Proteins at Nanogram Levels with Bordeaux Red Based on the Enhancement of Resonance Light Scattering [J]. Spectrochimica Acta Part A, 2006, 64 (3): 574-579.
[35] LI Y, DONG L J, WANG W P, et al. Flow Injection Analysis-Rayleigh Light Scattering Detection for Online Determination of Protein in Human Serum Sample [J]. Analytical Biochemistry, 2006,354 (1): 64-69.
[36] LIU S P, YANG Z, LIU Z F, et al. Resonance Rayleigh-scattering Method for the Determination of Proteins with Gold Nanoparticle Probe [J]. Analytical Biochemistry, 2006, 353 (1): 108-116.
[37] DONG L J, LI Y, ZHANG Y H,et al. A Flow Injection Sampling Resonance Light Scattering System for Total Protein Determination in Human Serum [J]. Spectrochimica Acta Part A, 2007, 66 (4-5): 1317-1322.
[38] BADDINI A L Q, CUNHA L E R, OLIVEIR A M C, et al. Determination of Total Protein in Hyperimmune Serum Samples by Near-infrared Spectrometry and Multivariate Calibration [J]. Analytical Biochemistry, 2010, 397 (2): 175-180.
[39] 金华丽，许春红，徐泽林.近红外光谱法测定小麦籽粒中的蛋白质含量[J].河南工业大学学报(自然科学版)，2010，31(6)：21-24.
[40] KHODABUX K, L’OMELETTE M S S, JHAUMEER-LAULLOO S, et al. Chemical and Near-infrared Determination of Moisture, Fat and Protein in Tuna Fishes [J]. Food Chemistry, 2007, 102(3): 669-675.
[41] ALMENDINGEN K, MELTZER H M, PEDERSEN J I, et al. Near Infrared Spectroscopy-a Potentially Useful Method for Rapid Determination of Fat and Protein Content in Homogenized Diets [J]. European Journal of Clinical Nutrition, 2000, 54(1): 20-23.
[42] XIN Q, LING H Z, LONG T J, et al. The Rapid Determination of Fat and Protein Content in Fresh raw Milk Using the Laser Light Scattering Technology [J]. Optics and Lasers in Engineering, 2006, 44 (8): 858-869.
[43] 朱向荣，单杨，李高阳，等.近红外光谱法快速测定液态奶中蛋白质和脂肪含量[J].食品科学，2011，32(12)：191-195.
[44] 王锡昌，陆烨，刘源.近红外光谱技术快速无损测定狭鳕鱼糜水分和蛋白质含量[J].食品科学，2010，31(16)：168-171.
[45] VAKNIN Y, GHANIM M, SAMRA S, et al. Predicting Jatropha Curcas Seed-oil Content, Oil Composition and Protein Content Using Near-infrared Spectroscopy-a Quick and Non-destructive Method [J]. Industrial Crops and Products, 2011, 34(1): 1029-1034.