食品科学 ›› 2013, Vol. 34 ›› Issue (11): 109-112.doi: 10.7506/spkx1002-6630-201311024

• 基础研究 • 上一篇    下一篇

马面鱼骨胶原多肽的理化特性及其抗氧化活性

杨 露,丁利君,蓝德安   

  1. 1.广东工业大学轻工化工学院,广东 广州 510006;2.广州市华琪生物科技有限公司,广东 广州 510220
  • 收稿日期:2012-03-16 修回日期:2013-04-11 出版日期:2013-06-15 发布日期:2013-06-03
  • 通讯作者: 丁利君 E-mail:ddddlj@foxmail.com
  • 基金资助:

    葡萄酒

Physicochemical Characterization and Antioxidant Properties of Collagen Polypeptide from Navodon septentionalis Bone

YANG Lu,DING Li-jun,LAN De-an   

  1. 1. Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology,Guangzhou 510006, China;2. Guangzhou Huaqi Biotechnology Co. Ltd., Guangzhou 510220, China
  • Received:2012-03-16 Revised:2013-04-11 Online:2013-06-15 Published:2013-06-03

摘要:

以马面鱼骨为原料,采用现代生物酶工程技术制得胶原蛋白多肽酶解液,经冷冻干燥,获取马面鱼骨胶原蛋白多肽粉。采用紫外吸收光谱分析,鱼骨中胶原蛋白多肽的最大吸收峰值约在波长220nm处,符合肽类基本特征;氨基酸自动分析仪测定鱼骨胶原蛋白多肽粉的氨基酸,其中羟脯氨酸含量约为3%。鱼骨胶原多肽液质量浓度60mg/mL的抗氧化效果和谷胱甘肽相当,25mg/mL时对·OH的清除率和DPPH自由基的清除率分别高达95.98%和97.36%。

关键词: 马面鱼骨, 胶原多肽, 氨基酸分析, MALDI-TOF, 抗氧化

Abstract:

In this study, lyophilized samples of collagen peptides were prepared from enzymatic hydrolysis of Navodon
septentionalis bone. Our analysis results showed that the peptides had maximum absorption at 220 nm and consisted of 3%
hydroxyproline. At 60 mg/mL, the peptides had antioxidant activity equivalent to that of GSH, and at 25 mg/mL, scavenged
95.98% of hydroxyl free radical and 97.36% of 1,1-diphenyl-2-picryhydrazyl (DPPH) free radical.

Key words: Navodon septentionalis bone, collagen polypeptide, amino analysis, MALDI-TOF, antioxidant activity

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