食品科学

• 工艺技术 • 上一篇    下一篇

黄参多糖提取工艺参数优化

罗光宏,李金燕,叶生宝,苏凤贤,王海蓉   

  1. 1.河西学院凯源生物技术开发中心,甘肃省微藻工程技术研究中心,甘肃 张掖 734000;
    2.河西学院农业与生物技术学院,甘肃 张掖 734000;3.温州科技职业学院,浙江 温州 325006
  • 出版日期:2013-12-25 发布日期:2014-01-03

Optimization of Process Parameters for the Extraction of Polysaccharides from Sphallerocarpus gracilis Roots

LUO Guang-hong,LI Jin-yan,YE Sheng-bao,SU Feng-xian,WANG Hai-rong   

  1. 1. Gansu Engineering Research Center for Microalgae, Kai-yuan Bio-tech Development Center, Hexi University, Zhangye 734000, China;
    2. College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China;
    3. Wenzhou Vocational College of Science and Technology, Wenzhou 325006, China
  • Online:2013-12-25 Published:2014-01-03

摘要:

以水提醇沉法提取黄参多糖,用硫酸-苯酚比色法测定黄参多糖含量。通过单因素试验和Box-Behnken试验,对黄参多糖提取工艺参数进行优化。结果表明:黄参多糖的最佳提取工艺为料液比1:26.7(g/mL)、浸提温度90℃、浸提时间67min、浸提2次,在此条件下,多糖得率为3.41%。

关键词: 黄参, 多糖, 参数优化, 响应面分析

Abstract:

Polysaccharides from the roots of Sphallerocarpus gracilis were extracted into water and precipitated with
ethanol, and then determined by the sulfuric acid-phenol method. One-factor-at-a-time and Box-Behnken experimental
designs were used to obtain the optimal extraction parameters for polysaccharides. Analysis of variance showed that solidto-
liquid ratio and extraction time as well as the quadratic terms of all four response factors tested had a significant effect on
the yield of polysaccharides. A regression equation with a correlation coefficient of 94.57% was established, which could fit
the experimental data at a 94.57% confidence interval showing the reliability of the predictive model. Using ridge analysis,
the optimal extraction parameters of polysaccharide were obtained as follows: extraction temperature of 90 ℃, extraction
duration of 67 min, solid-toliquid ratio of 1:26.7 and 2 repeated cycles of extraction. Under these conditions, the yield of
polysaccharides was 3.41%.

Key words: Sphallerocarpus gracilis, polysaccharide, parameter optimization, response surface methodology