食品科学 ›› 2020, Vol. 41 ›› Issue (3): 138-143.doi: 10.7506/spkx1002-6630-20181219-226

• 营养卫生 • 上一篇    下一篇

牛磺酸对抑郁症模型小鼠的预防性干预作用

袁静,闫晨静,周茜,牛佳卉,吴梦颖,王亚旭,王颉,赵文   

  1. (河北农业大学食品科技学院,河北省农产品加工工程技术中心,河北 保定 071001)
  • 出版日期:2020-02-15 发布日期:2020-02-26
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFD0901004); 河北省现代农业产业技术体系生猪创新团队资助项目(HBCT2018110205); 河北省食品科学与工程学科“双一流”建设资金项目(2016SPGCA18)

Interventional Effect of Taurine in Mouse Models of Depression

YUAN Jing, YAN Chenjing, ZHOU Qian, NIU Jiahui, WU Mengying, WANG Yaxu, WANG Jie, ZHAO Wen   

  1. (Hebei Agricultural Products Processing Engineering Technology Center, College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China)
  • Online:2020-02-15 Published:2020-02-26

摘要: 目的:研究牛磺酸对抑郁的干预作用。方法:采用行为绝望和利血平诱导的急性抑郁症两种小鼠模型。将模型小鼠随机分成6 组:阴性对照组、模型对照组、氟西汀对照组、3 个牛磺酸剂量组(0.5、1、2 g/kg mb),每组10 只。通过悬尾实验、强迫游泳实验、旷场实验建立的行为绝望小鼠模型进行研究,研究牛磺酸对小鼠到达行为绝望时间的影响;通过分析眼睑下垂、肛温、出圈率等行为学指标,血清中促肾上腺皮质激素(adreno-corticotropic hormone,ACTH)、脑源性神经营养因子(brain derived neurotrophic factor,BDNF)、皮质醇(cortisol,COR),脑组织中5-羟色胺(5-hydroxytryptamine,5-HT)、去甲肾上腺素(norepinephrine,NE)、多巴胺(dopamine,DA)、单胺氧化酶(monoamine oxidase,MAO)、促肾上腺皮质激素释放因子(corticotropin-releasing factor,CRF)等生化指标,研究牛磺酸对急性抑郁的干预作用。结果:牛磺酸显著性缩短了行为绝望模型小鼠悬尾不动时间(P<0.01)和强迫小鼠游泳不动时间(P<0.01),在旷场实验中,显著性升高水平、垂直运动次数(P<0.01),眼睑下垂度无显著性差异(P>0.05),显著升高抑郁小鼠脑组织5-HT、DA、NE水平(P<0.05),降低MAO活性(P<0.01),并升高BDNF含量(P>0.05),同时抑制CRF、ACTH、COR的过度分泌(P<0.01),并改善相关生化指标。结论:牛磺酸可能是通过改善模型动物的激素分泌,干预利血平诱导的急性抑郁症。

关键词: 牛磺酸, 利血平, 抑郁, 眼睑下垂, 出圈率

Abstract: Objective: To study the effect of taurine on depression-related behaviors and biochemical indexes in mouse models of depression. Methods: Two mouse models of behavioral despair or acute depression induced by reserpine, respectively were used for this study. The model mice were randomly divided into 6 groups: negative control, model control, fluoxetine control, and three taurine dose groups (0.5, 1 and 2 g/kg mb), with 10 animals in each group. The effect of taurine on the despair time of mice was investigated by tail suspension, and forced swimming and open field tests, and its effect on depression-related behaviors such as ptosis, rectal temperature and escape rate as well as depression-related biochemical indicators including (adreno-corticotropic hormone) ATCH, brain derived neurotrophic factor (BDNF) and cortisol (COR) in serum and 5-hydroxytryptamine (5-HT), norepinephrine (NE), dopamine (DA), monoamine oxidase (MAO) and corticotropin-releasing factor (CRF) in brain tissue was evaluated in the mouse model of acute depression. Results: Taurine significantly shortened the suspension time of the behavioral despair model mice (P < 0.01) and forced swimming time (P < 0.01). In the open field test, taurine significantly increased the level and number of vertical movements (P < 0.01), but resulted in no significant difference in ptosis rate (P > 0.05). Meanwhile, taurine significantly increased the levels of 5-HT, DA and NE in brain tissue of depressed mice (P < 0.05), decreased MAO activity (P < 0.01), augmented BDNF content (P > 0.05), inhibited excessive secretion of CRF, ACTH and COR (P < 0.01), and improved other relevant biochemical indicators. Conclusion: Taurine may be an effective interventional strategy for reserpine-induced acute depression by improving hormone secretion in model animals.

Key words: taurine, reserpine, depression, ptosis, escape rate

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